Houston Methodist. Leading Medicine.
Houston Methodist. Leading Medicine

Genomic Sequencing Core

To learn more or schedule resources for this Core, please login to iLab.

The core operates a core facility that is devoted to helping you keep on top of sequencing trends. 

Ion Torrent Personal Genome Machine

The genomic sequencing facility includes equipment for the processing of samples for Illumina and Ion Torrent sequencing analysis as well as Illumina bead array analysis.  This equipment now includes an Ion Torrent Personal Genome Machine (PGM), capable of producing whole-genome-scale sequence data in a single-day run cycle, as well as a Genome Analyzer IIx, which regularly produces in excess of 3.5 gigabases of sequence information per week-long sequencing run.  This equipment has been used for the sequencing of genomic DNA, Chromatin-IP DNA samples (ChIP-Seq), RNA samples (RNA-seq), microRNA and samples derived from novel experimental approaches. 

Supporting equipment includes an Illumina Cluster Station and a PCR workstation for the processing of samples.  We use an Eppendorf Epmotion 5075 robotics system for the automated processing of samples.  Our laboratory also has three realtime PCR machines (two Roche LightCycler 480 II and one Applice Biosystems 7900HT) for individual gene expression analysis.

Project Guidelines

To start a new project, we recommend that you contact us to discuss the details.  We can provide you with some useful information on what will be necessary to successfully complete your project, and we often able to provide some useful advise on how best to implement projects, based on our own extensive experience in this field.  This initial consultation is free and carries no obligation to continue your project with our center.

Sample submission:

Once you are ready to submit samples for your project, we will send you a sample submission form by email. 

Genomic Sequencing Core Location:

6767 Bertner Avenue
RIB 8th floor
Houston, Texas 77030

Contact: Doug Sieglaff

Current Projects

Metabolomics: The central emphasis of our lab is the study of the mechanisms by which genetic and epigenetic changes alter the metabolic profile of individuals, and the ways in which these processes can lead to metabolic disease states.  We are specifically interested in the mechanisms of action of nuclear hormone receptors, including thyroid receptor and PPARgamma.  Toward this end, we have used ChIP-Seq and RNA-seq to examine these mechanisms on a genome-wide scale.

Mechanisms of Cancer Initiation: Most current models attribute cancer initiation to genetic or epigenetic events.  However, years of investigation have demonstrated that these events are often complex and cryptic in nature.  Genome-wide techniques provide us with a new method with which to study these events on a global scale.  The projects at our center have provided new insight into these initiating events, using RNAseq and related techniques.

Pathogen Genomics: Next generation sequencing techniques have provided a valuable tool for the genomic analysis of unicellular pathogens.  Our center has used such sequencing strategies to successfully investigate outbreaks and demonstrate the utility of such approaches as novel diagnostic tools for their analysis.

Genomic Drug Profiling: Whole-genome analysis techniques have provided a valuable set of tools to investigate the effects of novel drugs on a genome-wide scale.  Our lab has pioneered the use of ChiP-Seq, RNA-seq and array-based methodologies to specifically investigate the effects of novel drugs on a genome-wide scale.  We are currently pursuing these techniques to determine the effects of novel therapeutic compounds in the activation of specific gene profiles and with distinct, tissue-specific expression patterns.