John P. Cooke

John P. Cooke, MD, PhD

Joseph C. “Rusty” Walter and Carole Walter Looke Presidential Distinguished Chair in Cardiovascular Disease Research, Department of Cardiovascular Sciences
Chair, Department of Cardiovascular Sciences
Professor of Cardiovascular Sciences, Academic Institute
Full Member, Research Institute
Director, Center for Cardiovascular Regeneration
Medical Director, Center for RNA Therapeutics
Houston Methodist
Weill Cornell Medical College


Center for Cardiovascular Regeneration


Biography

Dr. John P. Cooke is the Chair of the Department of Cardiovascular Sciences at the Houston Methodist Research Institute, Director of the Center for Cardiovascular Regeneration, and Medical Director of the RNA Therapeutics Program in the Houston Methodist DeBakey Heart and Vascular Center in Houston, Texas.

He trained in cardiovascular medicine and obtained a Ph.D. in physiology at the Mayo Clinic. He was recruited to Harvard Medical School as an assistant professor of medicine. In 1990, he was recruited to Stanford University to spearhead the program in vascular biology and medicine, and was appointed professor in the Division of Cardiovascular Medicine at Stanford University School of Medicine, and associate director of the Stanford Cardiovascular Institute until his recruitment to Houston Methodist in 2013.

Dr. Cooke has published over 500 research papers, position papers, reviews, book chapters and patents in the arena of vascular medicine and biology with over 30,000 citations. He has served on national and international committees that deal with cardiovascular diseases, including the American Heart Association, American College of Cardiology, Society for Vascular Medicine, and the National Heart, Lung and Blood Institute. He has served as president of the Society for Vascular Medicine, as a director of the American Board of Vascular Medicine, and as an associate editor of Vascular Medicine.

Description of Research

Dr. Cooke’s research program is focused on vascular regeneration, vascular cell identity and cell fate. The program is funded by grants from the National Institutes of Health, the American Heart Association, Cancer Prevention Research Institute, and industry.

The Cooke group aims to understand the mechanisms underlying epigenetic plasticity that are required for functional adaptation to cellular challenges.  Innate immune signaling causes global changes in the expression and activity of epigenetic modifiers with metabolic coupling that favors an open chromatin configuration.  The translational output of this work is vascular regeneration via therapeutic transdifferentiation using small molecules or mRNA. In his 25 years of translational vascular biology, Dr. Cooke first described and characterized the anti-atherogenic effects of endothelium-derived nitric oxide; the anti-angiogenic effect of the NO synthase inhibitor ADMA; the angiogenic pathway mediated by endothelial nicotinic acetylcholine receptors; the role for this pathway in states of pathological angiogenesis; and developed an antagonist of the pathway that was tested in clinical trials. His clinical research group has explored the use of angiogenic agents and adult stem cells in the treatment of peripheral arterial disease. More recently, his group has generated and characterized vascular cells diferentiated from iPSCs of patients with Progeria, to understand the role of telomere erosion in this condition of accelerated aging and vascular death. His group's applicaiton of mRNA encoding human telomerase to reverse aging in this condition, and other age-related diseases, is promising.

Areas Of Expertise

Regenerative medicine Stem cell Vascular disease Endothelium Telomeres and Aging
Education & Training

Clinical Fellowship, Mayo Graduate School of Medicine, Rochester, MN
Residency, Mayo Graduate School of Medicine, Rochester, MN
Internship, Mayo Graduate School of Medicine, Rochester, MN
MD, Wayne State Univ. School of Medicine
PhD, Mayo Graduate School of Medicine, Rochester, NY
Research Fellowship, Mayo Graduate School of Medicine, Rochester, NY
Publications

Emerging nanotechnologies in cardiovascular medicine
Grattoni, A & Cooke, JP 2022, , Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 39, 102472. https://doi.org/10.1016/j.nano.2021.102472

Nucleus-mitochondria positive feedback loop formed by ERK5 S496 phosphorylation-mediated poly (ADP-ribose) polymerase activation provokes persistent pro-inflammatory senescent phenotype and accelerates coronary atherosclerosis after chemo-radiation
Kotla, S, Zhang, A, Imanishi, M, Ko, KA, Lin, SH, Gi, YJ, Moczygemba, M, Isgandarova, S, Schadler, KL, Chung, C, Milgrom, SA, Banchs, J, Yusuf, SW, Amaya, DN, Guo, H, Thomas, TN, Shen, YH, Deswal, A, Herrmann, J, Kleinerman, ES, Entman, ML, Cooke, JP, Schifitto, G, Maggirwar, SB, McBeath, E, Gupte, AA, Krishnan, S, Patel, ZS, Yoon, Y, Burks, JK, Fujiwara, K, Brookes, PS, Le, N-T, Hamilton, DJ & Abe, J-I 2021, , Redox Biology, vol. 47, 102132, pp. 102132. https://doi.org/10.1016/j.redox.2021.102132

Fli1+ cells transcriptional analysis reveals an Lmo2-Prdm16 axis in angiogenesis
Matrone, G, Xia, B, Chen, K, Denvir, MA, Baker, AH & Cooke, JP 2021, , Proceedings of the National Academy of Sciences of the United States of America, vol. 118, no. 31, e2008559118. https://doi.org/10.1073/pnas.2008559118

Induced endothelial cells from peripheral arterial disease patients and neonatal fibroblasts have comparable angiogenic properties
Hywood, JD, Sadeghipour, S, Clayton, ZE, Yuan, J, Stubbs, C, Wong, JWT, Cooke, JP & Patel, S 2021, , PLoS ONE, vol. 16, no. 8 August, e0255075. https://doi.org/10.1371/journal.pone.0255075

Telomerase therapy reverses vascular senescence and extends lifespan in progeria mice
Mojiri, A, Walther, BK, Jiang, C, Matrone, G, Holgate, R, Xu, Q, Morales, E, Wang, G, Gu, J, Wang, R & Cooke, JP 2021, , European heart journal, vol. 42, no. 42, pp. 4352-4369. https://doi.org/10.1093/eurheartj/ehab547

Biomimetic and immunomodulatory therapeutics as an alternative to natural exosomes for vascular and cardiac applications
Villarreal-Leal, RA, Cooke, JP & Corradetti, B 2021, , Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 35, 102385, pp. 102385. https://doi.org/10.1016/j.nano.2021.102385

Mechanotransduction-on-chip: Vessel-chip model of endothelial YAP mechanobiology reveals matrix stiffness impedes shear response
Walther, BK, Rajeeva Pandian, NK, Gold, KA, Kiliç, ES, Sama, V, Gu, J, Gaharwar, AK, Guiseppi-Elie, A, Cooke, JP & Jain, A 2021, , Lab on a Chip, vol. 21, no. 9, pp. 1738-1751. https://doi.org/10.1039/d0lc01283a

RNA therapeutics for cardiovascular disease
Boada, C, Sukhovershin, R, Pettigrew, R & Cooke, JP 2021, , Current Opinion in Cardiology, vol. 36, no. 3, pp. 256-263. https://doi.org/10.1097/HCO.0000000000000850

The Limitless Future of RNA Therapeutics
Damase, TR, Sukhovershin, R, Boada, C, Taraballi, F, Pettigrew, RI & Cooke, JP 2021, , Frontiers in Bioengineering and Biotechnology, vol. 9, 628137. https://doi.org/10.3389/fbioe.2021.628137

mRNA-Enhanced Cell Therapy and Cardiovascular Regeneration
Chanda, PK, Sukhovershin, R & Cooke, JP 2021, , Cells, vol. 10, no. 1, 187, pp. 1-13. https://doi.org/10.3390/cells10010187

Biomimetic nano drug delivery carriers for treating cardiovascular diseases
Zinger, A, Cooke, JP & Taraballi, F 2021, , Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 33, 102360, pp. 102360. https://doi.org/10.1016/j.nano.2021.102360

Role of Endothelial and Mesenchymal Cell Transitions in Heart Failure and Recovery Thereafter
Wang, G, Cruz, AS, Youker, K, Marcos-Abdala, HG, Thandavarayan, RA, Cooke, JP, Torre-Amione, G, Chen, K & Bhimaraj, A 2021, , Frontiers in Genetics, vol. 11, 609262, pp. 609262. https://doi.org/10.3389/fgene.2020.609262

Endothelial Dysfunction-related Neurological Bleeds with Continuous Flow-Left Ventricular Assist Devices Measured by Digital Thermal Monitor
Ali, A, Uribe, C, Araujo-Gutierrez, R, Cruz-Solbes, AS, Marcos-Abdala, HG, Youker, KA, Guha, A, Torre-Amione, G, Nguyen, DT, Graviss, EA, Cooke, JP & Bhimaraj, A 2021, , ASAIO Journal, pp. 561-566. https://doi.org/10.1097/MAT.0000000000001281

New Directions in Therapeutic Angiogenesis and Arteriogenesis in Peripheral Arterial Disease
Annex, BH & Cooke, JP 2021, , Circulation Research, vol. 128, no. 12, pp. 1944-1957. https://doi.org/10.1161/CIRCRESAHA.121.318266

Endothelial Dysfunction-related Neurological Bleeds with Continuous Flow-Left Ventricular Assist Devices Measured by Digital Thermal Monitor
Ali, A, Uribe, C, Araujo-Gutierrez, R, Cruz-Solbes, AS, Marcos-Abdala, HG, Youker, KA, Guha, A, Torre-Amione, G, Nguyen, DT, Graviss, EA, Cooke, JP & Bhimaraj, A 2021, , ASAIO Journal, pp. 561-566. https://doi.org/10.1097/MAT.0000000000001281

MACMIC Reveals A Dual Role of CTCF in Epigenetic Regulation of Cell Identity Genes
Wang, G, Xia, B, Zhou, M, Lv, J, Zhao, D, Li, Y, Bu, Y, Wang, X, Cooke, JP, Cao, Q, Lee, MG, Zhang, L & Chen, K 2021, , Genomics, Proteomics and Bioinformatics, vol. 19, no. 1, pp. 140-153. https://doi.org/10.1016/j.gpb.2020.10.008

3D Bioprinted Multicellular Vascular Models
Gold, KA, Saha, B, Rajeeva Pandian, NK, Walther, BK, Palma, JA, Jo, J, Cooke, JP, Jain, A & Gaharwar, AK 2021, , Advanced Healthcare Materials, vol. 10, no. 21, 2101141. https://doi.org/10.1002/adhm.202101141

Senescence-Associated Secretory Phenotype as a Hinge Between Cardiovascular Diseases and Cancer
Banerjee, P, Kotla, S, Reddy Velatooru, L, Abe, RJ, Olmsted-Davis, E, Cooke, JP, Schadler, KL, Deswal, A, Herrmann, J, Lin, SH, Abe, JI & Le, N-T 2021, , Front Cardiovasc Med.

Microengineered Human Vein-Chip Recreates Venous Valve Architecture and Its Contribution to Thrombosis
Rajeeva Pandian, NK, Walther, BK, Suresh, R, Cooke, JP & Jain, A 2020, , Small, vol. 16, no. 49, 2003401. https://doi.org/10.1002/smll.202003401

Microengineered Human Vein-Chip Recreates Venous Valve Architecture and Its Contribution to Thrombosis
Rajeeva Pandian, NK, Walther, BK, Suresh, R, Cooke, JP & Jain, A 2020, , Small (Weinheim an der Bergstrasse, Germany), vol. 16, no. 49, pp. e2003401. https://doi.org/10.1002/smll.202003401