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Samuel Shelburne, MD

Assistant Affiliate Member, Research Institute
Houston Methodist


After graduating from University of Texas Medical Branch in Galveston, TX with high honors, Dr. Shelburne began his residency training at Baylor College of Medicine in Houston, TX. During his residency, Dr. Shelburne began his research career, first studying immune responses in patients undergoing HIV therapy, and then in the laboratory of Dr. Jim Musser where he began genetically profiling group A Streptococcus (GAS) during its interaction with human saliva. Dr. Shelburne identified a two component system that is activated during GAS colonization of the nasopharynx that controls the expression of several virulence factors and proteins involved in carbohydrate acquisition and metabolism. He has received several awards including an Outstanding Clinical Research Award and an Outstanding Resident Educator from the Baylor College of Medicine, and was awarded a Mentored Clinical Scientist Development Award from the NIH for his work on GAS.

Description of Research

Research in the Shelburne laboratory focuses on understanding the molecular basis of bacterial infections in humans, especially Gram-positive pathogens. Currently he is investigating the relationship between carbon-source utilization and virulence in group A Streptococcus (the “flesh-eating bacteria”). Dr. Shelburne’s laboratory utilizes such molecular techniques as whole genome sequencing, expression microarray analysis, isogenic mutant strain creation, and purified protein analysis to define the contribution of specific group A Streptococcus genes/proteins to disease.

An example of the research in the Shelburne laboratory is shown in the accompanying figure. Through expression microarray analysis, researchers in the Shelburne laboratory were able to elucidate a unique regulatory pathway linking nutrient utilization with virulence factor production. A long-term goal of the Shelburne laboratory is to elucidate novel aspects of bacterial pathogenesis that may be amenable to pharmaceutical intervention or prevention.

Areas Of Expertise

Group A Streptococcus Strep throat Carbohydrate metabolism
Education & Training

MS , Baylor College of Medicine

In vitro activity of ceftaroline and comparator agents against Gram-positive and Gram-negative clinical isolates from cancer patients
Rolston, KVI, Jamal, MA, Nesher, L, Shelburne, SA, Raad, I & Prince, RA 2017, International Journal of Antimicrobial Agents, vol 49, no. 4, pp. 416-421. DOI:

Erratum to: An integrative Bayesian Dirichlet-multinomial regression model for the analysis of taxonomic abundances in microbiome data [ BMC Bioinform. 18, (2017)(94)] DOI: 10.1186/s12859-017-1516-0
Wadsworth, WD, Argiento, R, Guindani, M, Galloway-Pena, J, Shelburne, SA & Vannucci, M 2017, BMC Bioinformatics, vol 18, no. 1, 185. DOI:

Characterization of oral and gut microbiome temporal variability in hospitalized cancer patients
Galloway-Peña, JR, Smith, DP, Sahasrabhojane, P, Wadsworth, WD, Fellman, BM, Ajami, NJ, Shpall, EJ, Daver, N, Guindani, M, Petrosino, JF, Kontoyiannis, DP & Shelburne, SA 2017, Genome Medicine, vol 9, no. 1, 21. DOI:

Identification and whole genome sequencing of the first case of Kosakonia radicincitans causing a human bloodstream infection
Bhatti, MD, Kalia, A, Sahasrabhojane, P, Kim, J, Greenberg, DE & Shelburne, SA 2017, Frontiers in Microbiology, vol 8, no. JAN, 62. DOI:

High Rates of Nonsusceptibility to Ceftazidime-avibactam and Identification of New Delhi Metallo-ß-lactamase Production in Enterobacteriaceae Bloodstream Infections at a Major Cancer Center
Aitken, SL, Tarrand, JJ, Deshpande, LM, Tverdek, FP, Jones, AL, Shelburne, SA, Prince, RA, Bhatti, MM, Rolston, KVI, Jones, RN, Castanheira, M & Chemaly, RF 2016, Clinical Infectious Diseases, vol 63, no. 7, pp. 954-958. DOI:

A Multi-Serotype Approach Clarifies the Catabolite Control Protein A Regulon in the Major Human Pathogen Group A Streptococcus
Debroy, S, Saldaña, M, Travisany, D, Montano, A, Galloway-Peña, J, Horstmann, N, Yao, H, González, M, Maass, A, Latorre, M & Shelburne, SA 2016, Scientific Reports, vol 6, 32442. DOI:

Successful treatment of bloodstream infection due to metallo-ß-lactamase-producing Stenotrophomonas maltophilia in a renal transplant patient
Mojica, MF, Ouellette, CP, Leber, A, Becknell, MB, Ardura, MI, Perez, F, Shimamura, M, Bonomo, RA, Aitken, SL & Shelburne, SA 2016, Antimicrobial Agents and Chemotherapy, vol 60, no. 9, pp. 5130-5134. DOI:

The role of the gastrointestinal microbiome in infectious complications during induction chemotherapy for acute myeloid leukemia
Galloway-Peña, JR, Smith, DP, Sahasrabhojane, P, Ajami, NJ, Wadsworth, WD, Daver, NG, Chemaly, RF, Marsh, L, Ghantoji, SS, Pemmaraju, N, Garcia-Manero, G, Rezvani, K, Alousi, AM, Wargo, JA, Shpall, EJ, Futreal, PA, Guindani, M, Petrosino, JF, Kontoyiannis, DP & Shelburne, SA 2016, Cancer, vol 122, no. 14, pp. 2186-2196. DOI:

Influence of Minimum Inhibitory Concentration in Clinical Outcomes of Enterococcus faecium Bacteremia Treated with Daptomycin: Is it Time to Change the Breakpoint?
Shukla, BS, Shelburne, S, Reyes, K, Kamboj, M, Lewis, JD, Rincon, SL, Reyes, J, Carvajal, LP, Panesso, D, Sifri, CD, Zervos, MJ, Pamer, EG, Tran, TT, Adachi, J, Munita, JM, Hasbun, R & Arias, CA 2016, Clinical Infectious Diseases, vol 62, no. 12, pp. 1514-1520. DOI:

Intergenic variable-number tandem-repeat polymorphism upstream of rocA alters toxin production and enhances virulence in Streptococcus pyogenes
Zhu, L, Olsen, RJ, Horstmann, N, Shelburne, SA, Fan, J, Hu, T & Musser, JM 2016, Infection and Immunity, vol 84, no. 7, pp. 2086-2093. DOI:

In vitro activity of dalbavancin and five comparator agents against common and uncommon Gram-positive organisms isolated from cancer patients
Rolston, KVI, Wang, W, Nesher, L, Shelburne, SA & Prince, RA 2016, Journal of Antibiotics, vol 69, no. 5, pp. 381-387. DOI:

Complete genome sequence of Streptococcus mitis strain SVGS_061 isolated from a neutropenic patient with viridans group streptococcal shock syndrome
Petrosyan, V, Holder, M, Ajami, NJ, Petrosino, JF, Sahasrabhojane, P, Thompson, EJ, Kalia, A & Shelburne, SA 2016, Genome Announcements, vol 4, no. 2, e00259-16. DOI:

Daptomycin non-susceptible Enterococcus faecium in leukemia patients: Role of prior daptomycin exposure
DiPippo, AJ, Tverdek, FP, Tarrand, JJ, Munita, JM, Tran, TT, Arias, CA, Shelburne, SA & Aitken, SL 2016, Journal of Infection. DOI:

Application of whole-genome sequencing to an unusual outbreak of invasive group a streptococcal disease
Galloway-Peña, J, Clement, ME, Kuinkel, BKS, Ruffin, F, Flores, AR, Levinson, H, Shelburne, SA, Moore, Z & Fowler, VG 2016, Open Forum Infectious Diseases, vol 3, no. 1, ofw042. DOI:

Implementation of a pan-genomic approach to investigate holobiont-infecting microbe interaction: A case report of a leukemic patient with invasive mucormycosis
Shelburne, SA, Ajami, NJ, Chibucos, MC, Beird, HC, Tarrand, J, Galloway-Peña, J, Albert, N, Chemaly, RF, Ghantoji, SS, Marsh, L, Pemmaraju, N, Andreeff, M, Shpall, EJ, Wargo, JA, Rezvani, K, Alousi, A, Bruno, VM, Futreal, PA, Petrosino, JF & Kontoyiannis, DP 2015, PLoS ONE, vol 10, no. 11, e0139851. DOI:

Regulatory rewiring confers serotype-specific hyper-virulence in the human pathogen group A Streptococcus
Miller, EW, Danger, JL, Ramalinga, AB, Horstmann, N, Shelburne, SA & Sumby, P 2015, Molecular Microbiology, vol 98, no. 3, pp. 473-489. DOI:

Emergence of serotype IV group B streptococcus adult invasive disease in Manitoba and saskatchewan, Canada, is driven by clonal sequence type 459 strains
Teatero, S, Athey, TBT, Van Caeseele, P, Horsman, G, Alexander, DC, Melano, RG, Li, A, Flores, AR, Shelburne, SA, McGeer, A, Demczuk, W, Martin, I & Fittipaldi, N 2015, Journal of Clinical Microbiology, vol 53, no. 9, pp. 2919-2926. DOI:

Sequence type 1 group B Streptococcus, an emerging cause of invasive disease in adults, evolves by small genetic changes
Flores, AR, Galloway-Peña, J, Sahasrabhojane, P, Saldaña, M, Yao, H, Su, X, Ajami, NJ, Holder, ME, Petrosino, JF, Thompson, E, Ros, IMY, Rosini, R, Grandi, G, Horstmann, N, Teatero, S, McGeer, A, Fittipaldi, N, Rappuoli, R, Baker, CJ & Shelburne, SA 2015, Proceedings of the National Academy of Sciences of the United States of America, vol 112, no. 20, pp. 6431-6436. DOI:

The identification and functional characterization of WxL proteins from Enterococcus faecium reveal surface proteins involved in extracellular matrix interactions
Galloway-Peña, JR, Liang, X, Singh, KV, Yadav, P, Chang, C, La Rosa, SL, Shelburne, S, Ton-That, H, Höök, M & Murray, BE 2015, Journal of Bacteriology, vol 197, no. 5, pp. 882-892. DOI:

Characterization of the effect of the histidine kinase CovS on response regulator phosphorylation in group a Streptococcus
Horstmann, N, Sahasrabhojane, P, Saldaña, M, Ajami, NJ, Flores, AR, Sumby, P, Liu, CG, Yao, H, Su, X, Thompson, E & Shelburne, SA 2015, Infection and Immunity, vol 83, no. 3, pp. 1068-1077. DOI: