Not found

Samuel Shelburne, MD

Assistant Affiliate Member, Research Institute
Houston Methodist


Biography

After graduating from University of Texas Medical Branch in Galveston, TX with high honors, Dr. Shelburne began his residency training at Baylor College of Medicine in Houston, TX. During his residency, Dr. Shelburne began his research career, first studying immune responses in patients undergoing HIV therapy, and then in the laboratory of Dr. Jim Musser where he began genetically profiling group A Streptococcus (GAS) during its interaction with human saliva. Dr. Shelburne identified a two component system that is activated during GAS colonization of the nasopharynx that controls the expression of several virulence factors and proteins involved in carbohydrate acquisition and metabolism. He has received several awards including an Outstanding Clinical Research Award and an Outstanding Resident Educator from the Baylor College of Medicine, and was awarded a Mentored Clinical Scientist Development Award from the NIH for his work on GAS.

Description of Research

Research in the Shelburne laboratory focuses on understanding the molecular basis of bacterial infections in humans, especially Gram-positive pathogens. Currently he is investigating the relationship between carbon-source utilization and virulence in group A Streptococcus (the “flesh-eating bacteria”). Dr. Shelburne’s laboratory utilizes such molecular techniques as whole genome sequencing, expression microarray analysis, isogenic mutant strain creation, and purified protein analysis to define the contribution of specific group A Streptococcus genes/proteins to disease.

An example of the research in the Shelburne laboratory is shown in the accompanying figure. Through expression microarray analysis, researchers in the Shelburne laboratory were able to elucidate a unique regulatory pathway linking nutrient utilization with virulence factor production. A long-term goal of the Shelburne laboratory is to elucidate novel aspects of bacterial pathogenesis that may be amenable to pharmaceutical intervention or prevention.

Areas Of Expertise

Group A Streptococcus Strep throat Carbohydrate metabolism
Education & Training

, Baylor College of Medicine
Publications

High Rates of Nonsusceptibility to Ceftazidime-avibactam and Identification of New Delhi Metallo-ß-lactamase Production in Enterobacteriaceae Bloodstream Infections at a Major Cancer Center
Aitken, SL, Tarrand, JJ, Deshpande, LM, Tverdek, FP, Jones, AL, Shelburne, SA, Prince, RA, Bhatti, MM, Rolston, KVI, Jones, RN, Castanheira, M & Chemaly, RF 2016, Clinical Infectious Diseases, vol 63, no. 7, pp. 954-958. DOI:

Successful treatment of bloodstream infection due to metallo-ß-lactamase-producing Stenotrophomonas maltophilia in a renal transplant patient
Mojica, MF, Ouellette, CP, Leber, A, Becknell, MB, Ardura, MI, Perez, F, Shimamura, M, Bonomo, RA, Aitken, SL & Shelburne, SA 2016, Antimicrobial Agents and Chemotherapy, vol 60, no. 9, pp. 5130-5134. DOI:

A Multi-Serotype Approach Clarifies the Catabolite Control Protein A Regulon in the Major Human Pathogen Group A Streptococcus
Debroy, S, Saldaña, M, Travisany, D, Montano, A, Galloway-Peña, J, Horstmann, N, Yao, H, González, M, Maass, A, Latorre, M & Shelburne, SA 2016, Scientific Reports, vol 6, 32442. DOI:

The role of the gastrointestinal microbiome in infectious complications during induction chemotherapy for acute myeloid leukemia
Galloway-Peña, JR, Smith, DP, Sahasrabhojane, P, Ajami, NJ, Wadsworth, WD, Daver, NG, Chemaly, RF, Marsh, L, Ghantoji, SS, Pemmaraju, N, Garcia-Manero, G, Rezvani, K, Alousi, AM, Wargo, JA, Shpall, EJ, Futreal, PA, Guindani, M, Petrosino, JF, Kontoyiannis, DP & Shelburne, SA 2016, Cancer, vol 122, no. 14, pp. 2186-2196. DOI:

Influence of Minimum Inhibitory Concentration in Clinical Outcomes of Enterococcus faecium Bacteremia Treated with Daptomycin: Is it Time to Change the Breakpoint?
Shukla, BS, Shelburne, S, Reyes, K, Kamboj, M, Lewis, JD, Rincon, SL, Reyes, J, Carvajal, LP, Panesso, D, Sifri, CD, Zervos, MJ, Pamer, EG, Tran, TT, Adachi, J, Munita, JM, Hasbun, R & Arias, CA 2016, Clinical Infectious Diseases, vol 62, no. 12, pp. 1514-1520. DOI:

Intergenic variable-number tandem-repeat polymorphism upstream of rocA alters toxin production and enhances virulence in Streptococcus pyogenes
Zhu, L, Olsen, RJ, Horstmann, N, Shelburne, SA, Fan, J, Hu, T & Musser, JM 2016, Infection and Immunity, vol 84, no. 7, pp. 2086-2093. DOI:

In vitro activity of dalbavancin and five comparator agents against common and uncommon Gram-positive organisms isolated from cancer patients
Rolston, KVI, Wang, W, Nesher, L, Shelburne, SA & Prince, RA 2016, Journal of Antibiotics, vol 69, no. 5, pp. 381-387. DOI:

The role of the gastrointestinal microbiome in infectious complications during induction chemotherapy for acute myeloid leukemia
Galloway-Peña, JR, Smith, DP, Sahasrabhojane, P, Ajami, NJ, Wadsworth, WD, Daver, NG, Chemaly, RF, Marsh, L, Ghantoji, SS, Pemmaraju, N, Garcia-Manero, G, Rezvani, K, Alousi, AM, Wargo, JA, Shpall, EJ, Futreal, PA, Guindani, M, Petrosino, JF, Kontoyiannis, DP & Shelburne, SA 2016, Cancer.

Intergenic variable-number tandem-repeat polymorphism upstream of rocA alters toxin production and enhances virulence in Streptococcus pyogenes
Zhu, L, Olsen, RJ, Horstmann, N, Shelburne, SA, Fan, J, Hu, Y & Musser, JM 2016, Infection and Immunity, vol 84, no. 7, pp. 2086-2093.

Application of whole-genome sequencing to an unusual outbreak of invasive group a streptococcal disease
Galloway-Peña, J, Clement, ME, Kuinkel, BKS, Ruffin, F, Flores, AR, Levinson, H, Shelburne, SA, Moore, Z & Fowler, VG 2016, Open Forum Infectious Diseases, vol 3, no. 1, ofw042. DOI:

Daptomycin non-susceptible Enterococcus faecium in leukemia patients: Role of prior daptomycin exposure
DiPippo, AJ, Tverdek, FP, Tarrand, JJ, Munita, JM, Tran, TT, Arias, CA, Shelburne, SA & Aitken, SL 2016, Journal of Infection. DOI:

Implementation of a pan-genomic approach to investigate holobiont-infecting microbe interaction: A case report of a leukemic patient with invasive mucormycosis
Shelburne, SA, Ajami, NJ, Chibucos, MC, Beird, HC, Tarrand, J, Galloway-Peña, J, Albert, N, Chemaly, RF, Ghantoji, SS, Marsh, L, Pemmaraju, N, Andreeff, M, Shpall, EJ, Wargo, JA, Rezvani, K, Alousi, A, Bruno, VM, Futreal, PA, Petrosino, JF & Kontoyiannis, DP 2015, PLoS ONE, vol 10, no. 11, e0139851. DOI:

Regulatory rewiring confers serotype-specific hyper-virulence in the human pathogen group A Streptococcus
Miller, EW, Danger, JL, Ramalinga, AB, Horstmann, N, Shelburne, SA & Sumby, P 2015, Molecular Microbiology, vol 98, no. 3, pp. 473-489. DOI:

Emergence of serotype IV group B streptococcus adult invasive disease in Manitoba and saskatchewan, Canada, is driven by clonal sequence type 459 strains
Teatero, S, Athey, TBT, Van Caeseele, P, Horsman, G, Alexander, DC, Melano, RG, Li, A, Flores, AR, Shelburne, SA, McGeer, A, Demczuk, W, Martin, I & Fittipaldi, N 2015, Journal of Clinical Microbiology, vol 53, no. 9, pp. 2919-2926. DOI:

Sequence type 1 group B Streptococcus, an emerging cause of invasive disease in adults, evolves by small genetic changes
Flores, AR, Galloway-Peña, J, Sahasrabhojane, P, Saldaña, M, Yao, H, Su, X, Ajami, NJ, Holder, ME, Petrosino, JF, Thompson, E, Ros, IMY, Rosini, R, Grandi, G, Horstmann, N, Teatero, S, McGeer, A, Fittipaldi, N, Rappuoli, R, Baker, CJ & Shelburne, SA 2015, Proceedings of the National Academy of Sciences of the United States of America, vol 112, no. 20, pp. 6431-6436. DOI:

Characterization of the effect of the histidine kinase CovS on response regulator phosphorylation in group a Streptococcus
Horstmann, N, Sahasrabhojane, P, Saldaña, M, Ajami, NJ, Flores, AR, Sumby, P, Liu, CG, Yao, H, Su, X, Thompson, E & Shelburne, SA 2015, Infection and Immunity, vol 83, no. 3, pp. 1068-1077. DOI:

The identification and functional characterization of WxL proteins from Enterococcus faecium reveal surface proteins involved in extracellular matrix interactions
Galloway-Peña, JR, Liang, X, Singh, KV, Yadav, P, Chang, C, La Rosa, SL, Shelburne, S, Ton-That, H, Höök, M & Murray, BE 2015, Journal of Bacteriology, vol 197, no. 5, pp. 882-892. DOI:

The identification and functional characterization of WxL proteins from Enterococcus faecium reveal surface proteins involved in extracellular matrix interactions
Galloway-Peña, JR, Liang, X, Singh, KV, Yadav, P, Chang, C, La Rosa, SL, Shelburne, S, Ton-That, H, Höök, M & Murray, BE 2015, Journal of Bacteriology, vol 197, no. 5, pp. 882-892. DOI:

Development and validation of a clinical model to predict the presence of ß-lactam resistance in viridans group streptococci causing bacteremia in neutropenic cancer patients
Shelburne, SA, Lasky, RE, Sahasrabhojane, P, Tarrand, JT & Rolston, KVI 2014, Clinical Infectious Diseases, vol 59, no. 2, pp. 223-230. DOI:

Molecular characterization of an invasive phenotype of group a streptococcus arising during human infection using whole genome sequencing of multiple isolates from the same patient
Flores, AR, Sahasrabhojane, P, Saldaña, M, Galloway-Peña, J, Olsen, RJ, Musser, JM & Shelburne, SA 2014, Journal of Infectious Diseases, vol 209, no. 10, pp. 1520-1523. DOI: