Ping-Ying Pan

Ping-Ying Pan, PhD

Professor of Oncology, Academic Institute
Full Member, Research Institute
Associate Director, Center for Immunotherapy Research
Houston Methodist


Ping-Ying Pan, Ph.D., is Associate Director of Cancer Immunotherapy Research Center at Houston Methodist Research Institute. Prior to joining Houston Methodist, he was an Associate Professor in the Department of Oncological Sciences in the Icahn School of Medicine at Mount Sinai in New York. He obtained his Ph.D. from The University of Texas Health Science Center at San Antonio, Texas in 1996 and finished his postdoctoral training at Department of Medicine, Columbia University. In 2000, he became a senior research associate at Carl C. Icahn Institute for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine (currently Icahn School of Medicine at Mount Sinai, aka ISMMS), and rose to the rank of Associate Professor of the Department of Oncological Sciences, ISMMS in 2009. He has made significant contributions to the fields of cancer immunology and immunotherapy. Dr. Pan has been focusing on studying the mechanisms by which cancer evades immune responses in the tumor microenvironment (TME), more specifically the contribution of myeloid derived suppressor cells (MDSC) to tumor progression. His group is one of the first to demonstrate that MDSCs can act as a “Trojan Horse” in the delivery of oncolytic viruses or therapeutic agents used in direct tumor targeting, thereby preventing tumor metastases. His lab has also demonstrated the use of MDSC, as a cell-based tolerogenic therapy, to suppress unwanted immune responses, such as autoimmunity and graft-versus-host diseases (GVHD), while still retaining graft verse leukemia (GVL) function. More recently, Dr. Pan identified a potential role of myeloid inhibitory receptors in the maintenance of tumor-initiating cells (TIC) and radio/chemo-resistance of colon cancer. Dr. Pan has served on national and international grant review panels and as a reviewer for multiple international journals. His laboratory has published important, high-impact research articles and his research has been continuously supported by NIH grants, private foundations, and pharmaceutical companies.

Description of Research

Graft-versus-host disease (GVHD) is the leading cause of morbidity and mortality following allogeneic hematopoietic stem cell transplantation, an established therapy for patients with hematological malignancies. Current strategies to diminish GVHD include T-cell depletion and immunosuppressive drugs, which are associated with an increased risk of tumor relapse, opportunistic infection, and/or toxicity. Novel approaches acting intrinsically on the immune system are clearly needed. The focus of my research is to harness the suppressive function of myeloid-derived suppressor cells (MDSCs) for the treatment/prevention of graft-versus-host diseases (GVHDs) and to study the mechanisms underlying the differential regulation of GVHD and graft-versus-leukemia/lymphoma (GVL) activities by myeloid-derived suppressor cells (MDSCs). The ultimate goal of my research is to identify signaling pathways and targets that regulate the functional activities (immunostimulatory versus immunosuppressive) of MDSCs. The information garnered from these studies can be utilized for the treatment of cancer, autoimmune, and viral infectious diseases.

Myeloid-derived suppressor cells (MDSCs) suppress T-cell responses through multiple mechanisms. We demonstrated that MDSCs induced antigen specific T regulatory cells and T-cell anergy in vivo. The unique suppressive function of MDSCs endows them with potential clinical merits. While it is widely accepted that MDSCs offer an appealing target for therapeutic intervention in cancer treatment, a growing body of evidence suggests that MDSCs per se have great therapeutic potential in the pathologic settings where deleterious or excessive immune responses need to be avoided or reduced. We have demonstrated that upon adoptive transfer, MDSCs can efficiently prevent graft-versus-host disease (GVHD) without compromising the graft-versus-leukemia effects (GVL) following allogeneic hematopoietic stem cell transplantation (allo-HSCT) in murine models. Recently we also demonstrated that MDSCs acquire M1 or M2 functional macrophage phenotypes through agonism and antagonism of myeloid inhibitory receptor signaling, which may facilitate the development of antitumor responses or mediate immune suppression and Treg activation, respectively. We hypothesize that: (i) The functional phenotype of MDSC can be modulated by PIR-BL ligation and (ii) the presence of MDSCs with a persistent M2 functional phenotype may be sufficient to prevent GHVD and retain GVL ability.

Cancer stem/initiating cells and chemoresistance

Cancer initiating cells exhibit distinct markers and are highly tumorigenic. Several research groups have successfully isolated colorectal cancer initiating cells (CCICs) based on distinct cell-surface markers. Conventional therapies, including chemotherapies and radiotherapies, target the bulk population of rapidly proliferating colorectal cancer cells. Although a large proportion of tumor mass is eradicated, therapy-resistant CCICs remain and can differentiate into colon cancer cells. Repopulation of the tumor over time by the progenies of CCICs ultimately results in a more aggressive phenotype than the initial pretreated malignancy. With 56,500 fatalities per year, colorectal cancer is second only to lung cancer as a cause of cancer deaths in the USA. Each year, 150,000 to 160,000 new cases are diagnosed of which 10 to 20% already have liver metastases. About 70% of all colorectal cancer patients eventually develop liver metastases. Hepatic resection is currently the only form of treatment that offers long-term survival, with 5-year survival rates ranging from 25% to 39%. Using the current selection criteria for hepatectomy, only 10% to 20% of all patients are candidates for curative operation. The prognosis for the remaining patients is grim with palliative chemotherapy and symptomatic treatments being the only available options. There are no reliable treatment modalities for controlling cancer recurrence after chemotherapy. Our laboratory has found that myeloid inhibitory receptors play an important role in controlling the “stemness” of colon cancer initiating cells and induction of epithelial-mesenchymal transition (EMT). We found that activation of myeloid inhibitory receptor promoted acquisition of an M2 (alternative) macrophage functional phenotype by myeloid-derived suppressor cell, induced “stemness” phenotypes of colon tumor cells, and enhanced metastases. We hypothesize that myeloid inhibitory receptor activation can induce the phenotype of colon cancer initiating cell and EMT, thereby facilitating the development of chemotherapy resistance. Our study will identify the molecular determinants of myeloid inhibitory receptor-mediated signaling in control of CCIC and EMT phenotypes, and provide new information for devising a novel therapeutic modality for refractory colorectal cancer.

Research Directions:

1. Study the regulatory pathways of MDSC function and the associated effects on GVHD.

2. Study the mechanism of MDSC mediated differential regulation of alloimmunity (GVHD vs. GVL) in pre-existing tumor models.

3. Study the effects of myeloid inhibitory receptor activation through agonistic antibodies on MDSC as related to MDSC suppressive function, inhibition of GVHD, and the corresponding signaling regulation.

4. Study the mechanism and effects of MDSC mediated regulation of GVHD vs. GVL through myeloid inhibitory receptor activation in mouse GVHD models and in a human xenograft NSG mouse model.

5. Study whether the GVT effect in allogeneic bone marrow transplantation can be used to eradicate metastatic cancers that cannot be successfully eliminated by operation, chemotherapy, or radiation therapy.

6. Study the molecular mechanisms underlying the regulation of colon cancer stemness, EMT, and chemoresistance by myeloid inhibitory receptors ectopically expressed by tumor cells.

7. Determine the effect of myeloid inhibitory receptor antagonism on stemness and EMT phenotype of colon cancer.

8. Assess the therapeutic efficacy of myeloid inhibitory receptor antagonism in combination with chemotherapies for the treatment of colon cancer in xenograft mouse models of human colon cancer.


Tocilizumab overcomes chemotherapy resistance in mesenchymal stem-like breast cancer by negating autocrine IL-1A induction of IL-6
Chung, AW, Kozielski, AJ, Qian, W, Zhou, J, Anselme, AC, Chan, AA, Pan, PY, Lee, DJ & Chang, JC 2022, , npj Breast Cancer, vol. 8, no. 1, 30.

Dedifferentiation-mediated stem cell niche maintenance in early-stage ductal carcinoma in situ progression: insights from a multiscale modeling study
Butner, JD, Dogra, P, Chung, C, Ruiz-Ramírez, J, Nizzero, S, Plodinec, M, Li, X, Pan, P-Y, Chen, S-H, Cristini, V, Ozpolat, B, Calin, GA & Wang, Z 2022, , Cell death & disease, vol. 13, no. 5, pp. 485.

A phase 1/2 clinical trial of the nitric oxide synthase inhibitor L-NMMA and taxane for treating chemoresistant triple-negative breast cancer
Chung, AW, Anand, K, Anselme, AC, Chan, AA, Gupta, N, Venta, LA, Schwartz, MR, Qian, W, Xu, Y, Zhang, L, Kuhn, J, Patel, T, Rodriguez, AA, Belcheva, A, Darcourt, J, Ensor, J, Bernicker, E, Pan, PY, Chen, SH, Lee, DJ, Niravath, PA & Chang, JC 2021, , Science translational medicine, vol. 13, no. 624, abj5070.

The Sympathetic Nervous System Modulates Cancer Vaccine Activity through Monocyte-Derived Cells
Hinkle, L, Liu, Y, Meng, C, Chen, Z, Mai, J, Zhang, L, Xu, Y, Pan, P-Y, Chen, S-H & Shen, H 2021, , Journal of immunology (Baltimore, Md. : 1950).

Early prediction of clinical response to checkpoint inhibitor therapy in human solid tumors through mathematical modeling
Butner, JD, Martin, GV, Wang, Z, Corradetti, B, Ferrari, M, Esnaola, N, Chung, C, Hong, DS, Welsh, JW, Hasegawa, N, Mittendorf, EA, Curley, SA, Chen, S-H, Pan, P-Y, Libutti, SK, Ganesan, S, Sidman, RL, Pasqualini, R, Arap, W, Koay, EJ & Cristini, V 2021, , eLife, vol. 10.

Early prediction of clinical response to checkpoint inhibitor therapy in human solid tumors through mathematical modeling
Butner, JD, Martin, GV, Wang, Z, Corradetti, B, Ferrari, M, Esnaola, N, Chung, C, Hong, DS, Welsh, JW, Hasegawa, N, Mittendorf, EA, Curley, SA, Chen, SH, Pan, PY, Libutti, SK, Ganesan, S, Sidman, RL, Pasqualini, R, Arap, W, Koay, EJ & Cristini, V 2021, , eLife, vol. 10, e70130.

Myeloid-derived suppressor cells as cellular immunotherapy in transplantation and autoimmune diseases
Zhang, J, Hodges, A, Chen, SH & Pan, PY 2021, , Cellular Immunology, vol. 362, 104300.

Corrigendum: Analyzing One Cell at a TIME: Analysis of Myeloid Cell Contributions in the Tumor Immune Microenvironment (Frontiers in Immunology, (2020), 11, (1842), 10.3389/fimmu.2020.01842)
Davidov, V, Jensen, G, Mai, S, Chen, SH & Pan, PY 2021, , Frontiers in immunology, vol. 11, 645213.

Nutrient supplements from selected botanicals mediated immune modulation of the tumor microenvironment and antitumor mechanism
Chen, HM, Sun, L, Pan, PY, Wang, LH & Chen, SH 2021, , Cancer Immunology, Immunotherapy, vol. 70, no. 12, pp. 3435-3449.

Virus-Mimic mRNA Vaccine for Cancer Treatment
Meng, C, Chen, Z, Mai, J, Shi, Q, Tian, S, Hinkle, L, Li, J, Zhang, Z, Ramirez, M, Zhang, L, Xu, Y, Zhang, J, Pan, PY, Chen, SH, Li, H & Shen, H 2021, , advanced therapeutics, vol. 4, no. 11, 2100144.

Analyzing One Cell at a TIME: Analysis of Myeloid Cell Contributions in the Tumor Immune Microenvironment
Davidov, V, Jensen, G, Mai, S, Chen, SH & Pan, PY 2020, , Frontiers in immunology, vol. 11, 1842.

The mechanistic study behind suppression of GVHD while retaining GVL activities by myeloid-derived suppressor cells
Zhang, J, Chen, HM, Ma, G, Zhou, Z, Raulet, D, Rivera, AL, Chen, SH & Pan, PY 2019, , Leukemia, vol. 33, no. 8, pp. 2078-2089.

Blocking immunoinhibitory receptor LILRB2 reprograms tumor-associated myeloid cells and promotes antitumor immunity
Chen, H-M, van der Touw, W, Wang, YS, Kang, K, Mai, S, Zhang, J, Alsina-Beauchamp, D, Duty, JA, Mungamuri, SK, Zhang, B, Moran, T, Flavell, R, Aaronson, S, Hu, H-M, Arase, H, Ramanathan, S, Flores, R, Pan, P-Y & Chen, S-H 2018, , The Journal of clinical investigation, vol. 128, no. 12, pp. 5647-5662.

Glatiramer acetate enhances myeloid-derived suppressor cell function via recognition of paired Ig-like receptor B
Van Der Touw, W, Kang, K, Luan, Y, Ma, G, Mai, S, Qin, L, Bian, G, Zhang, R, Mungamuri, SK, Hu, HM, Zhang, CC, Aaronson, SA, Feldmann, M, Yang, WC, Chen, SH & Pan, PY 2018, , Journal of Immunology, vol. 201, no. 6, pp. 1727-1734.

LILRB receptor-mediated regulation of myeloid cell maturation and function
van der Touw, W, Chen, HM, Pan, PY & Chen, SH 2017, , Cancer Immunology, Immunotherapy, vol. 66, no. 8, pp. 1079-1087.

Leukocyte immunoglobulin-like receptors in human diseases: An overview of their distribution, function, and potential application for immunotherapies
Zhang, J, Mai, S, Chen, H-M, Kang, K, Li, XC, Chen, SH & Pan, PY 2017, , Journal of Leukocyte Biology, vol. 102, no. 2, pp. 351-360.

Myeloid-derived suppressor cells as an immune parameter in patients with concurrent sunitinib and stereotactic body radiotherapy
Chen, HM, Ma, G, Gildener-Leapman, N, Eisenstein, S, Coakley, BA, Ozao, J, Mandeli, J, Divino, C, Schwartz, M, Sung, M, Ferris, R, Kao, J, Wang, LH, Pan, PY, Ko, EC & Chen, SH 2015, , Clinical Cancer Research, vol. 21, no. 18, pp. 4073-4085.

Myeloid-derived suppressor cells as a vehicle for tumor-specific oncolytic viral therapy
Eisenstein, S, Coakley, BA, Briley-Saebo, K, Ma, G, Chen, HM, Meseck, M, Ward, S, Divino, C, Woo, S, Chen, SH & Pan, PY 2013, , Cancer research, vol. 73, no. 16, pp. 5003-5015.

Polarization and reprogramming of myeloid-derived suppressor cells
Yang, WC, Ma, G, Chen, SH & Pan, PY 2013, , Journal of molecular cell biology, vol. 5, no. 3, pp. 207-209.

Myeloid-derived suppressor cells as a trojan horse: A cellular vehicle for the delivery of oncolytic viruses
Pan, PY, Chen, HM & Chen, SH 2013, , OncoImmunology, vol. 2, no. 8, e25083.