Longhou Fang, PhD

Associate Professor of Cardiovascular Sciences, Institute for Academic Medicine
Associate Member, Research Institute
Houston Methodist
Weill Cornell Medical College

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NETWORK

Biography

The Fang lab employ zebrafish and mouse models to studying the role of lipid metabolism in angiogenesis and lymphangiogenesis. During his postdoctoral studies at UC San Diego, Dr. Fang discovered that apolipoprotein (apoA-I) binding protein (AIBP) controls angiogenesis via regulation of cholesterol homeostasis.Based upon the findings, the Fang laboratory will be focusing on the role of AIBP-regulated lipid metabolism in angiogenesis and lymphangiogenesis. We will use a plethora of disease models such as heart hypertrophy, heart ischemia and reperfusion, peripheral artery ischemia and reperfusion, corneal angiogenesis, and tumorigenesis in mice as well as in zebrafish heart regeneration. Both zebrafish and mouse model will be used to study the role of AIBP in lymphangiogenesis.

Areas Of Expertise

Angiogenesis Lymphangiogenesis hematopoiesis cholesterol metabolism

Education & Training

Postdoctoral Associate, University of California, San Diego

PhD, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences Chinese Acade

BS, Anhui Normal University

Publications

AIBP-mediated cholesterol efflux instructs hematopoietic stem and progenitor cell fate

Gu, Q, Yang, X, Lv, J, Zhang, J, Xia, B, Kim, JD, Wang, R, Xiong, F, Meng, S, Clements, TP, Tandon, B, Wagner, DS, Diaz, MF, Wenzel, PL, Miller, YI, Traver, D, Cooke, JP, Li, W, Zon, LI, Chen, K, Bai, Y & Fang, L 2019, Science, pp. eaav1749. https://doi.org/10.1126/science.aav1749

TBX20 Regulates Angiogenesis Through the Prokineticin 2-Prokineticin Receptor 1 Pathway

Meng, S, Gu, Q, Yang, X, Lv, J, Owusu, I, Matrone, G, Chen, K, Cooke, JP & Fang, L 2018, Circulation, vol. 138, no. 9, pp. 913-928. https://doi.org/10.1161/CIRCULATIONAHA.118.033939

AIBP augments cholesterol efflux from alveolar macrophages to surfactant and reduces acute lung inflammation

Choi, SH, Wallace, AM, Schneider, DA, Burg, E, Kim, J, Alekseeva, E, Ubags, ND, Cool, CD, Fang, L, Suratt, BT & Miller, YI 2018, JCI insight, vol. 3, no. 16. https://doi.org/10.1172/jci.insight.120519

AIBP protects against metabolic abnormalities and atherosclerosis

Schneider, DA, Choi, SH, Agatisa-Boyle, C, Zhu, L, Kim, J, Pattison, J, Sears, DD, Gordts, PLSM, Fang, L & Miller, YI 2018, Journal of Lipid Research, vol. 59, no. 5, pp. 854-863. https://doi.org/10.1194/jlr.M083618

A minimally invasive approach to induce myocardial infarction in mice without thoracotomy

Sun, Q, Wang, KK, Pan, M, Zhou, JP, Qiu, XT, Wang, ZY, Yang, Z, Chen, Y, Shen, H, Gu, QL, Fang, L, Zhang, GG & Bai, YP 2018, Journal of Cellular and Molecular Medicine. https://doi.org/10.1111/jcmm.13708

Lmo2 (LIM-Domain-Only 2) Modulates Sphk1 (Sphingosine Kinase) and Promotes Endothelial Cell Migration

Matrone, G, Meng, S, Gu, Q, Lv, J, Fang, L, Chen, K & Cooke, JP 2017, Arteriosclerosis, thrombosis, and vascular biology, vol. 37, no. 10, pp. 1860-1868. https://doi.org/10.1161/ATVBAHA.117.309609

AIBP Limits Angiogenesis Thorough ?-Secretase-Mediated Upregulation of Notch Signaling

Mao, R, Meng, S, Gu, Q, Araujo-Gutierrez, R, Kumar, S, Yan, Q, Almazan, F, Youker, KA, Fu, Y, Pownall, HJ, Cooke, JP, Miller, YI & Fang, L 2017, Circulation research. https://doi.org/10.1161/CIRCRESAHA.116.309754

AIBP: A Novel Molecule at the Interface of Cholesterol Transport, Angiogenesis, and Atherosclerosis

Zhu, L & Fang, L 2015, Methodist DeBakey Cardiovascular Journal, vol. 11, no. 3, pp. 160-165. https://doi.org/10.14797/mdcj-11-3-160

Polo-like kinase 2 regulates angiogenic sprouting and blood vessel development

Yang, H, Fang, L, Zhan, R, Hegarty, JM, Ren, J, Hsiai, TK, Gleeson, JG, Miller, YI, Trejo, J & Chi, NC 2015, Developmental Biology, vol. 404, no. 2, pp. 49-60. https://doi.org/10.1016/j.ydbio.2015.05.011

Apoc2 loss-of-function zebrafish mutant as a genetic model of hyperlipidemia

Liu, C, Gates, KP, Fang, L, Amar, MJ, Schneider, DA, Geng, H, Huang, W, Kim, J, Pattison, J, Zhang, J, Witztum, JL, Remaley, AT, Dong, PD & Miller, YI 2015, Disease models & mechanisms, vol. 8, no. 8, pp. 989-998. https://doi.org/10.1242/dmm.019836

Oxidative stress activates endothelial innate immunity via sterol regulatory element binding protein 2 (SREBP2) transactivation of MicroRNA-92a

Chen, Z, Wen, L, Martin, M, Hsu, CY, Fang, L, Lin, FM, Lin, TY, Geary, MJ, Geary, GG, Zhao, Y, Johnson, DA, Chen, JW, Lin, SJ, Chien, S, Huang, HD, Miller, YI, Huang, PH & Shyy, JYJ 2015, Circulation, vol. 131, no. 9, pp. 805-814. https://doi.org/10.1161/CIRCULATIONAHA.114.013675

Zebrafish models of dyslipidemia: Relevance to atherosclerosis and angiogenesis

Fang, L, Liu, C & Miller, YI 2014, Translational Research, vol. 163, no. 2, pp. 99-108. https://doi.org/10.1016/j.trsl.2013.09.004

Targeted cholesterol efflux

Fang, L & Miller, YI 2013, Cell Cycle, vol. 12, no. 21, pp. 3345-3346. https://doi.org/10.4161/cc.26401

Control of angiogenesis by AIBP-mediated cholesterol efflux

Fang, L, Choi, SH, Baek, JS, Liu, C, Almazan, F, Ulrich, F, Wiesner, P, Taleb, A, Deer, E, Pattison, J, Torres-Vázquez, J, Li, AC & Miller, YI 2013, Nature, vol. 498, no. 7452, pp. 118-122. https://doi.org/10.1038/nature12166

Emerging applications for zebrafish as a model organism to study oxidative mechanisms and their roles in inflammation and vascular accumulation of oxidized lipids

Fang, L & Miller, YI 2012, Free Radical Biology and Medicine, vol. 53, no. 7, pp. 1411-1420. https://doi.org/10.1016/j.freeradbiomed.2012.08.004

Ezetimibe and simvastatin reduce cholesterol levels in zebrafish larvae fed a high-cholesterol diet

Baek, JS, Fang, L, Li, AC & Miller, YI 2012, Cholesterol, vol. 2012, 564705. https://doi.org/10.1155/2012/564705

In vivo visualization and attenuation of oxidized lipid accumulation in hypercholesterolemic zebrafish

Fang, L, Green, SR, Baek, JS, Lee, SH, Ellett, F, Deer, E, Lieschke, GJ, Witztum, JL, Tsimikas, S & Miller, YI 2011, Journal of Clinical Investigation, vol. 121, no. 12, pp. 4861-4869. https://doi.org/10.1172/JCI57755

Oxidation-specific epitopes are danger-associated molecular patterns recognized by pattern recognition receptors of innate immunity

Miller, YI, Choi, SH, Wiesner, P, Fang, L, Harkewicz, R, Hartvigsen, K, Boullier, A, Gonen, A, Diehl, CJ, Que, X, Montano, E, Shaw, PX, Tsimikas, S, Binder, CJ & Witztum, JL 2011, Circulation research, vol. 108, no. 2, pp. 235-248. https://doi.org/10.1161/CIRCRESAHA.110.223875

Oxidized cholesteryl esters and phospholipids in zebrafish larvae fed a high cholesterol diet: Macrophage binding and activation

Fang, L, Harkewicz, R, Hartvigsen, K, Wiesner, P, Choi, SH, Almazan, F, Pattison, J, Deer, E, Sayaphupha, T, Dennis, EA, Witztum, JL, Tsimikas, S & Miller, YI 2010, Journal of Biological Chemistry, vol. 285, no. 42, pp. 32343-32351. https://doi.org/10.1074/jbc.M110.137257

Lipoprotein modification and macrophage uptake: Role of pathologic cholesterol transport in atherogenesis

Miller, YI, Choi, SH, Fang, L & Tsimikas, S 2010, Sub-cellular biochemistry, vol. 51, pp. 229-251. https://doi.org/10.1007/978-90-481-8622-8_8

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