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Longhou Fang, PhD

Assistant Professor of Cardiovascular Sciences, Institute for Academic Medicine
Assistant Member, Research Institute
Houston Methodist
Weill Cornell Medical College


Biography

The Fang lab employ zebrafish and mouse models to studying the role of lipid metabolism in angiogenesis and lymphangiogenesis. During his postdoctoral studies at UC San Diego, Dr. Fang discovered that apolipoprotein (apoA-I) binding protein (AIBP) controls angiogenesis via regulation of cholesterol homeostasis.Based upon the findings, the Fang laboratory will be focusing on the role of AIBP-regulated lipid metabolism in angiogenesis and lymphangiogenesis. We will use a plethora of disease models such as heart hypertrophy, heart ischemia and reperfusion, peripheral artery ischemia and reperfusion, corneal angiogenesis, and tumorigenesis in mice as well as in zebrafish heart regeneration. Both zebrafish and mouse model will be used to study the role of AIBP in lymphangiogenesis.

Areas Of Expertise

Angiogenesis Lymphangiogenesis hematopoiesis cholesterol metabolism
Education & Training

Postdoctoral Associate, University of California, San Diego
PhD, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences Chinese Acade
BS, Anhui Normal University
Publications

AIBP protects against metabolic abnormalities and atherosclerosis
Schneider, DA, Choi, SH, Agatisa-Boyle, C, Zhu, L, Kim, J, Pattison, J, Sears, DD, Gordts, PLSM, Fang, L & Miller, YI 2018, Journal of Lipid Research, vol 59, no. 5, pp. 854-863. DOI: 10.1194/jlr.M083618

Lmo2 (LIM-Domain-Only 2) Modulates Sphk1 (Sphingosine Kinase) and Promotes Endothelial Cell Migration
Matrone, G, Meng, S, Gu, Q, Lv, J, Fang, L, Chen, K & Cooke, JP 2017, Arteriosclerosis, Thrombosis, and Vascular Biology, vol 37, no. 10, pp. 1860-1868. DOI: 10.1161/ATVBAHA.117.309609

AIBP Limits Angiogenesis Thorough ?-Secretase-Mediated Upregulation of Notch Signaling
Mao, R, Meng, S, Gu, Q, Araujo-Gutierrez, R, Kumar, S, Yan, Q, Almazan, F, Youker, KA, Fu, Y, Pownall, HJ, Cooke, JP, Miller, YI & Fang, L 2017, Circulation Research. DOI: 10.1161/CIRCRESAHA.116.309754

AIBP:: A Novel Molecule at the Interface of Cholesterol Transport, Angiogenesis, and Atherosclerosis
Zhu, L & Fang, L 2015, Methodist DeBakey Cardiovascular Journal, vol 11, no. 3, pp. 160-165. DOI: 10.14797/mdcj-11-3-160

Polo-like kinase 2 regulates angiogenic sprouting and blood vessel development
Yang, H, Fang, L, Zhan, R, Hegarty, JM, Ren, J, Hsiai, TK, Gleeson, JG, Miller, YI, Trejo, J & Chi, NC 2015, Developmental Biology, vol 404, no. 2, pp. 49-60. DOI: 10.1016/j.ydbio.2015.05.011

Apoc2 loss-of-function zebrafish mutant as a genetic model of hyperlipidemia
Liu, C, Gates, KP, Fang, L, Amar, MJ, Schneider, DA, Geng, H, Huang, W, Kim, J, Pattison, J, Zhang, J, Witztum, JL, Remaley, AT, Dong, PD & Miller, YI 2015, Disease models & mechanisms, vol 8, no. 8, pp. 989-998. DOI: 10.1242/dmm.019836

Oxidative stress activates endothelial innate immunity via sterol regulatory element binding protein 2 (SREBP2) transactivation of MicroRNA-92a
Chen, Z, Wen, L, Martin, M, Hsu, CY, Fang, L, Lin, FM, Lin, TY, Geary, MJ, Geary, GG, Zhao, Y, Johnson, DA, Chen, JW, Lin, SJ, Chien, S, Huang, HD, Miller, YI, Huang, PH & Shyy, JYJ 2015, Circulation, vol 131, no. 9, pp. 805-814. DOI: 10.1161/CIRCULATIONAHA.114.013675

Zebrafish models of dyslipidemia: Relevance to atherosclerosis and angiogenesis
Fang, L, Liu, C & Miller, YI 2014, Translational Research, vol 163, no. 2, pp. 99-108. DOI: 10.1016/j.trsl.2013.09.004

Targeted cholesterol efflux
Fang, L & Miller, YI 2013, Cell Cycle, vol 12, no. 21, pp. 3345-3346. DOI: 10.4161/cc.26401

Control of angiogenesis by AIBP-mediated cholesterol efflux
Fang, L, Choi, SH, Baek, JS, Liu, C, Almazan, F, Ulrich, F, Wiesner, P, Taleb, A, Deer, E, Pattison, J, Torres-Vázquez, J, Li, AC & Miller, YI 2013, Nature, vol 498, no. 7452, pp. 118-122. DOI: 10.1038/nature12166

Emerging applications for zebrafish as a model organism to study oxidative mechanisms and their roles in inflammation and vascular accumulation of oxidized lipids
Fang, L & Miller, YI 2012, Free Radical Biology and Medicine, vol 53, no. 7, pp. 1411-1420. DOI: 10.1016/j.freeradbiomed.2012.08.004

Ezetimibe and simvastatin reduce cholesterol levels in zebrafish larvae fed a high-cholesterol diet
Baek, JS, Fang, L, Li, AC & Miller, YI 2012, Cholesterol, vol 2012, 564705. DOI: 10.1155/2012/564705

In vivo visualization and attenuation of oxidized lipid accumulation in hypercholesterolemic zebrafish
Fang, L, Green, SR, Baek, JS, Lee, SH, Ellett, F, Deer, E, Lieschke, GJ, Witztum, JL, Tsimikas, S & Miller, YI 2011, Journal of Clinical Investigation, vol 121, no. 12, pp. 4861-4869. DOI: 10.1172/JCI57755

Oxidation-specific epitopes are danger-associated molecular patterns recognized by pattern recognition receptors of innate immunity
Miller, YI, Choi, SH, Wiesner, P, Fang, L, Harkewicz, R, Hartvigsen, K, Boullier, A, Gonen, A, Diehl, CJ, Que, X, Montano, E, Shaw, PX, Tsimikas, S, Binder, CJ & Witztum, JL 2011, Circulation Research, vol 108, no. 2, pp. 235-248. DOI: 10.1161/CIRCRESAHA.110.223875

Oxidized cholesteryl esters and phospholipids in zebrafish larvae fed a high cholesterol diet: Macrophage binding and activation
Fang, L, Harkewicz, R, Hartvigsen, K, Wiesner, P, Choi, SH, Almazan, F, Pattison, J, Deer, E, Sayaphupha, T, Dennis, EA, Witztum, JL, Tsimikas, S & Miller, YI 2010, Journal of Biological Chemistry, vol 285, no. 42, pp. 32343-32351. DOI: 10.1074/jbc.M110.137257

Lipoprotein modification and macrophage uptake: Role of pathologic cholesterol transport in atherogenesis
Miller, YI, Choi, SH, Fang, L & Tsimikas, S 2010, Sub-cellular biochemistry, vol 51, pp. 229-251. DOI: 10.1007/978-90-481-8622-8_8

Proteomic, functional and motif-based analysis of C-terminal Src kinase-interacting proteins
Yang, G, Li, Q, Ren, S, Lu, X, Fang, L, Zhou, W, Zhang, F, Xu, F, Zhang, Z, Zeng, R, Lottspeich, F & Chen, Z 2009, Proteomics, vol 9, no. 21, pp. 4944-4961. DOI: 10.1002/pmic.200800762

Toll-Like Receptor-4 and Lipoprotein Accumulation in Macrophages
Miller, YI, Choi, SH, Fang, L & Harkewicz, R 2009, Trends in Cardiovascular Medicine, vol 19, no. 7, pp. 227-232. DOI: 10.1016/j.tcm.2010.02.001

Vascular lipid accumulation, lipoprotein oxidation, and macrophage lipid uptake in hypercholesterolemic zebrafish
Stoletov, K, Fang, L, Choi, SH, Hartvigsen, K, Hansen, LF, Hall, C, Pattison, J, Juliano, J, Miller, ER, Almazan, F, Crosier, P, Witztum, JL, Klemke, RL & Miller, YI 2009, Circulation Research, vol 104, no. 8, pp. 952-960. DOI: 10.1161/CIRCRESAHA.108.189803

Proteome identification of binding-partners interacting with cell polarity protein Par3 in Jurkat cells
Zhou, Y, Fang, L, Du, D, Zhou, W, Feng, X, Chen, J, Zhang, Z & Chen, Z 2008, Acta Biochimica et Biophysica Sinica, vol 40, no. 8, pp. 729-739. DOI: 10.1111/j.1745-7270.2008.00452.x

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