Jin Wang

Jin Wang, PhD

Professor of Transplant Immunology in Surgery, Academic Institute
Full Member, Research Institute
Houston Methodist
Weill Cornell Medical College


jinwang@houstonmethodist.org
Description of Research

Research projects in the laboratory are focused on three areas: 1) To elucidate the molecular mechanisms for the regulation of mitochondrial autophagy; 2) To study the molecular mechanisms for immunological memory against infections; and 3) To develop a cure for HIV infection by the SECH approach through selective elimination of host cells harboring replication-competent HIV:

 

1. Autophagy is critical for mitochondrial quality control through the clearance of dysfunctional mitochondria. Autophagy is also important for the maintenance of mitochondrial homeostasis and the regulation of cellular metabolic functions. We have identified novel signaling complexes that regulate the specificity for mitochondrial autophagy by mass spectrometry. Ongoing studies aim to understand the molecular mechanisms for the specific recognition of dysfunctional or surplus mitochondria for degradation by autophagy. How selective mitochondrial autophagy regulates diverse cellular functions and protects the cellular fitness and longevity are being studied.

 

2. The generation of immunological memory by forming immune memory cells against pathogens is essential for the success of vaccines. We are studying the molecular mechanisms governing the formation and maintenance of immune memory cells. We have discovered an essential role for autophagy in the long-term maintenance of memory B cells against influenza. We aim to characterize the molecular mechanisms for immunological memory to facilitate the development of more effective vaccines against epidemic and pandemic infections.

 

3. The RNA genome of HIV is reverse-transcribed into DNA and integrated into the genome of host cells, resulting in persistent infections that are difficult to clear. We have developed a strategy to eradicate HIV infection by selective elimination of host cells harboring replication competent HIV (SECH). The SECH approach combines viral reactivation with induction of cell death and inhibition of autophagy to specifically delete host cells capable of producing HIV. SECH can clear HIV-1 infection in humanized mice,  and in PBMCs from HIV-1 patients. We will further investigate the efficacy and safety of SECH for HIV eradication in preclinical studies, and develop SECH as a therapeutic approach for treating people living with HIV. 

Areas Of Expertise

Immunological memory Mitochondrial autophagy Memory T cells Memory B cells HIV cure
Education & Training

Postdoctoral Associate, NIH
PhD, University of Southern California
Publications

Protection of Quiescence and Longevity of IgG Memory B Cells by Mitochondrial Autophagy
Kodali, S, Li, M, Budai, MM, Chen, M & Wang, J 2022, , Journal of immunology (Baltimore, Md. : 1950), vol. 208, no. 5, pp. 1085-1098. https://doi.org/10.4049/jimmunol.2100969

Clearance of HIV-1 or SIV reservoirs by promotion of apoptosis and inhibition of autophagy: Targeting intracellular molecules in cure-directed strategies
Chen, M, Li, M, Budai, MM, Rice, AP, Kimata, JT, Mohan, M & Wang, J 2022, , Journal of Leukocyte Biology. https://doi.org/10.1002/JLB.4MR0222-606

A recombinant bovine adenoviral mucosal vaccine expressing mycobacterial antigen-85B generates robust protection against tuberculosis in mice
Khan, A, Sayedahmed, EE, Singh, VK, Mishra, A, Dorta-Estremera, S, Nookala, S, Canaday, DH, Chen, M, Wang, J, Sastry, KJ, Mittal, SK & Jagannath, C 2021, , Cell Reports Medicine, vol. 2, no. 8, 100372, pp. 100372. https://doi.org/10.1016/j.xcrm.2021.100372

Dependence on Autophagy for Autoreactive Memory B Cells in the Development of Pristane-Induced Lupus
Jang, A, Sharp, R, Wang, J, Feng, Y, Wang, J & Chen, M 2021, , Frontiers in immunology, vol. 12, 701066, pp. 1-14. https://doi.org/10.3389/fimmu.2021.701066

Author Correction: Clearance of HIV infection by selective elimination of host cells capable of producing HIV (Nature Communications, (2020), 11, 1, (4051), 10.1038/s41467-020-17753-w)
Li, M, Liu, W, Bauch, T, Graviss, EA, Arduino, RC, Kimata, JT, Chen, M & Wang, J 2020, , Nature Communications, vol. 11, no. 1, 6281. https://doi.org/10.1038/s41467-020-20218-9

Clearance of HIV infection by selective elimination of host cells capable of producing HIV
Li, M, Liu, W, Bauch, T, Graviss, EA, Arduino, RC, Kimata, JT, Chen, M & Wang, J 2020, , Nature Communications, vol. 11, no. 1, 4051. https://doi.org/10.1038/s41467-020-17753-w

Maintenance of Germinal Center B cells by Caspase-9 through Promotion of Apoptosis and Inhibition of Necroptosis.
Zhang, J, Kodali, S, Chen, M & Wang, J 2020, , Journal of Immunology, vol. 205, no. 1, pp. 113-120. https://doi.org/10.4049/jimmunol.2000359

Metabolic Reprogramming in CD8+ T Cells During Acute Viral Infections
Gupta, SS, Wang, J & Chen, M 2020, , Frontiers in immunology, vol. 11, pp. 1013. https://doi.org/10.3389/fimmu.2020.01013

An autophagy-inducing and TLR-2 activating BCG vaccine induces a robust protection against tuberculosis in mice
Khan, A, Bakhru, P, Saikolappan, S, Das, K, Soudani, E, Singh, CR, Estrella, JL, Zhang, D, Pasare, C, Ma, Y, Sun, J, Wang, J, Hunter, RL, Tony Eissa, N, Dhandayuthapani, S & Jagannath, C 2019, , npj Vaccines, vol. 4, no. 1, 34, pp. 34. https://doi.org/10.1038/s41541-019-0122-8

NIX-Mediated Mitophagy Promotes Effector Memory Formation in Antigen-Specific CD8+ T Cells
Gupta, SS, Sharp, R, Hofferek, C, Kuai, L, Dorn, GW, Wang, J & Chen, M 2019, , Cell Reports, vol. 29, no. 7, pp. 1862-1877.e7. https://doi.org/10.1016/j.celrep.2019.10.032

Role of c-Jun terminal kinase (JNK) activation in influenza A virus-induced autophagy and replication
Zhang, J, Ruan, T, Sheng, T, Wang, J, Sun, J, Wang, J, Prinz, RA, Peng, D, Liu, X & Xu, X 2019, , Virology, vol. 526, pp. 1-12. https://doi.org/10.1016/j.virol.2018.09.020

Atg5 Supports R. australis Infection in Macrophages in vitro and in vivo
Bechelli, J, Vergara, L, Smalley, C, Buzhdygan, TP, Bender, S, Zhang, W, Liu, Y, Popov, VL, Wang, J, Garg, N, Hwang, S, Walker, DH & Fang, R 2018, , Infection and Immunity, pp. IAI.00651-18. https://doi.org/10.1128/IAI.00651-18

Increased Immunogenicity Through Autophagy
Jagannath, C, Khan, A & Wang, J 2018, . in MA Hayat (ed.), Immunology. vol. 1, Academic Press, pp. 35-54. https://doi.org/10.1016/B978-0-12-809819-6.00004-6

TRIM29 promotes DNA virus infections by inhibiting innate immune response
Xing, J, Zhang, A, Zhang, H, Wang, J, Li, XC, Zeng, M-S & Zhang, Z 2017, , Nature Communications, vol. 8, no. 1, 945. https://doi.org/10.1038/s41467-017-00101-w

TRIM29 promotes DNA virus infections by inhibiting innate immune response
Xing, J, Zhang, A, Zhang, H, Wang, J, Li, XC, Zeng, M-S & Zhang, Z 2017, , Nature Communications, vol. 8, no. 1, 945. https://doi.org/10.1038/s41467-017-00101-w

Ablation of Transcription Factor IRF4 Promotes Transplant Acceptance by Driving Allogenic CD4+ T Cell Dysfunction
Wu, J, Zhang, H, Shi, X, Xiao, X, Fan, Y, Minze, LJ, Wang, J, Ghobrial, RM, Xia, J, Sciammas, R, Li, XC & Chen, W 2017, , Immunity. https://doi.org/10.1016/j.immuni.2017.11.003

Regulation of B cell fate, survival, and function by mitochondria and autophagy
Sandoval, H, Kodali, S & Wang, J 2017, , Mitochondrion. https://doi.org/10.1016/j.mito.2017.11.005

Supramolecular Peptide Nanofibers Engage Mechanisms of Autophagy in Antigen-Presenting Cells
Rudra, JS, Khan, A, Clover, TM, Endsley, JJ, Zloza, A, Wang, J & Jagannath, C 2017, , ACS Omega, vol. 2, no. 12, pp. 9136-9143. https://doi.org/10.1021/acsomega.7b00525, https://doi.org/10.1021/acsomega.7b00525

Challenges and strategies for the eradication of the HIV reservoir
Kimata, JT, Rice, AP & Wang, J 2016, , Current Opinion in Immunology, vol. 42, pp. 65-70. https://doi.org/10.1016/j.coi.2016.05.015

Autophagy in Host Defense Against Viruses
Wang, J & Chen, M 2016, . in Autophagy Networks in Inflammation. Springer, Cham, pp. 185-199. https://doi.org/10.1007/978-3-319-30079-5_10