Equipment

Here is a closer look at our microscopy and other imaging tools for cell and tissue research available at the Advanced Cellular & Tissue Microscopy Core. We offer a range of cutting-edge tools to meet your needs for tissue and cell imaging and examination.

 

Fluo View™ 1000 Confocal Microscope (R6-411)

The Olympus FV1000 is a next-generation imaging system designed for high-resolution, confocal observation of both fixed and living cells. The FV1000 offers advances in confocal system performance while providing the speed and sensitivity required for live cell imaging with minimal risk of damage to living specimens. In addition, the FV1000 offers a revolutionary synchronized laser scanning system called the SIM Scanner. While one laser stimulates, the second laser simultaneously provides high-resolution imaging. This coordination of laser stimulation and imaging makes the FV1000 an ideal choice for FRAP, FLIP and photo-activation.

The FV1000 includes five lasers: 405 Laser Diode; 458, 488, 515 mArgon; 543 HeNe; 633 HeNe; and Near-infrared LD748.

 

Live Cell Confocal Image System (R6-411)

The system consists of an IX81 automated inverted microscope, SRV CCD Digital Camera and CO2/humidity/temperature control incubator. It delivers an excellent environment for live cell phase contrast, differential interference contrast (DIC) and various fluorescent imaging scenarios. The filters sets are for DAPI, Hoechst and AMCA excitation; for FITC/EGFP/BODIPY/FUO3DIO excitation; and for TEXAS RED and CY5. The objectives are 4X, 10X, 20X, 40X and 40X, 60X and 100X resolutions for oil immersion microscopy.

 

Live Cell Spinning Disk Confocal Image System (R6-411)

A spinning-disk (Nipkow disk) confocal microscope uses a series of moving pinholes on a disk to scan spot of light. Since a series of pinholes scans an area in parallel, each pinhole is allowed to hover over a specific area for a longer amount of time thereby reducing the excitation energy needed to illuminate a sample when compared with laser scanning microscopes. Decreased excitation energy reduces phototoxicity and photobleaching of a sample, which makes it ideal for imaging live cells or organisms. This system offers fully motorized disk control so a computer can easily engage the disk into the light path and select wavelengths through the included filter changer; this also makes it easier to switch between confocal and wide-field techniques. Image formation is obtained from an EM-CCD camera that allows full frame images to be acquired at up to 15 frames per second. The DSU is excellent for live-cell applications where speed of acquisition and minimal phototoxicity is paramount.

 

IncuCyte® S3 Live-Cell Imaging System (R6-411)

IncuCyte allows you to place a microscope inside your incubator, taking advantage of an asset you already own as well as eliminating any potential problems that may result from disturbing cells during the observation process. This IncuCyte approach makes long-term kinetic imaging convenient and affordable. It provides a more efficient and thorough set of solutions for long-term, live-cell imaging. The IncuCyte is designed with increased productivity and supports hundreds of standard varieties of cell culture vessels, including 96- and 384-well plates, T-flasks, dishes and micro-slides. With three configurable trays, you can mix and match multiple vessels and even monitor different experiments running concurrently. The IncuCyte automatically acquires and analyzes HD phase, and fluorescent (GFP and RFP) images of living cells with the option of using a 4x, 10x, or 20x objective. Coupled with the image acquisition is an analysis system that enables real-time measurements of cell health, proliferation, and function. Application specific processing and analysis is available for, but not limited to, cell migration/invasion, chemotaxis, monitoring spheroids, angiogenesis, and neurite tracking.

 

ImageXpress Micro High Content Screening Widefield Microscope (R6-417)

The ImageXpress Micro is a fully-integrated wide-filed imaging system for automatic acquisition and data analysis for high content cell-based screening system. It will fully satisfy the demanding need of drug discovery as well as phenotypic screening in biological research such as small molecules, nano particles or RNAi.

  • Accelerates screening to > 10 million cells per hour, up to 5 channels imaging acquisition simultaneously
  • Compatible with 6-, 24-, 96- and 384-format multi-well plate types and standard slides
  • Environmental control provides both capabilities for one-time fixed and kinetic live cell screening
  • Robotic fluidics option provides the compatibility of adding/exchanging solutions without taking the plates out

 

Regular Inverted BF and Fluorescent Microscope (R6-412)

The IX81 begins with a unique two-tiered, V-shaped optical design that provides tremendous versatility without frame modifications and bright images with minimal reflections. An optional six-position fluorescence filter turret accepts a unique analyzer holder for switching between DIC/polarized light and fluorescence observations. With its built-in motorized Z-axis drive, six-position nosepiece and light path selector, the IX81 is ready for multi-wavelength, advanced fluorescence and de-convolution techniques.

 

Regular Upright BF and Fluorescent Microscope (R6-411)

The BX61’s completely modular frame and optical design provides a flexible, fast, reliable and precise upright BF/fluorescent imaging system. It has been recently upgraded with reflecting imaging.

 

Nikon A1 Confocal Imaging System (R9-317)

The Nikon A1 Confocal Imaging System has a Hybrid Confocal Scan Head that enhances the optical efficiency and minimizes light loss. It can perform ultra-high-speed imaging at 420 fps (512x32 pixels) using a resonant scanner with a resonance frequency of 7.8kHz; the system provides fast spectral imaging at 16 fps (512x64 pixels) and real-time spectral unmixing. With average light transmission of 98 percent, it can deliver up to a 30 percent increase in fluorescence detection efficiency.

 

Laser Capture Micro-dissection (LCM, Arcturus) (R6-412)

This Laser Capture Micro-dissection (LCM) method is extraordinarily gentle and ideal for micro-dissection of single cells or small numbers of cells. The ArcturusXT™ micro-dissection instruments each combine LCM and UV laser cutting for ultimate micro-dissection flexibility. These instruments remove the guesswork from the micro-dissection process by allowing researchers to maintain custody of the sample throughout the experiment, ensuring that only the desired material has been collected.

 

ImageStreamX High Resolution Microscopy in Flow (R6-412)

The ImageStreamX combines the high-speed capture of high-resolution imagery with detailed quantitation to create a statistically robust microscopy platform for a wide range of cell analysis applications. ImageStreamX produces up to 12 high resolution images of each cell directly in flow, at rates exceeding 1,000 cells per second with the fluorescence sensitivity of the best conventional flow cytometers. These breakthrough capabilities allow you to quantitate cellular morphology and the intensity and location of fluorescent probes on, in or between cells, even in rare sub-populations and highly heterogeneous samples.

 

With this system, you can image cells directly in suspension with the resolution of a 60X microscope and the fluorescence sensitivity of the best flow cytometers. You can analyze highly heterogeneous samples and rare cell sub-populations at speeds exceeding 1,000 cells per second, as well as perform phenotypic and functional studies at the same time using up to five lasers and 12 images per cell. The system will help you quantitate virtually anything you can see using the IDEAS® software package’s numerous pre-defined fluorescence and morphologic parameters.

 

Spero® Infrared Chemical Imaging Microscope (R6-417)

Until recently, high resolution Infrared spectroscopic imaging of biological systems was performed with Fourier Transform Infrared (FTIR) spectrometer systems which required extremely long data collection times. The Daylight Solutions Spero infrared chemical imaging micro-spectrometer is a totally unique system. It provides rapid and precise spectral imaging based on Infrared spectroscopy by employing Quantum Cascade Laser (QCL) light sources and extremely high resolution infrared imaging cameras operating at room temperature. The Daylight Solutions Spero system is the only commercial QCL microscope available. The large format camera offers 14X higher pixel density per area than detectors used on FTIR spectrometers. This camera and QCL combination provide large area spectral imaging at 1.4 um per pixel resolution at rates over 20 times faster than FTIR based imaging systems. For example a single frequency image of a 1 cm square tissue sample can be imaged in approximately 5 minutes with Spero verses approximately 6 hours with an interferometer based FTIR microscope system.

 

Research Stereo Microscope (R6-417)

The SZX16 is designed for advanced research and is suitable for oblique and brightfield illumination. With an easy to use carousel the option to view fluorescence using DAPI, GFP, RFP, and Cy7 filters is available. Its maximum numerical aperture (NA) of 0.3 produces a resolution of 900 linepairs per millimeter, enabling more sample information to be gained efficiently. The large zoom ratio of 16.4:1, combined with the comprehensive range of parfocal objectives with excellent working distances, allows easy switching between a macro- and micro-view. Imaging of the whole organism down to fine microscopic and individual cell structures becomes an easy task with an optical system that provides a natural, distortion-free view.

 

EVOS™ FL Auto Imaging System (R6-417)

The EVOS® FL Auto Imaging System is a fully-automated, digital, inverted multi-channel fluorescence and transmitted light imaging system with outstanding workflow efficiency. Designed to meet demanding requirements over a broad range of applications, the EVOS® FL Auto system supports high-resolution mosaic tiling, multi-position well scanning, cell counting with thresholding, and time-lapse studies. The intuitive interface, proprietary light cubes, dual cameras, precision automated stage and parfocal optical system enables you to produce publication quality images in seconds.

 

Peripheral Equipment

We offer peripheral equipment to support our microscope and imaging systems:

  • Live cell microscopy environmental control system
  • Tissue culture incubator (37°C with 5 percent CO2) for short-term storage
  • Dell Precision 390 workstation
  • Olympus DP-BSW image capture and control software
  • Olympus LSM Software package
  • Intelligent Imaging Innovations’ SlideBook Software package for 2-D and 3-D imaging on PC computer platforms

 

For customized software development of specialized bioassays, contact Dr. Stephen Wong.

 

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