Here are some manufacturer resources that provide introductory explanations of optical microscopy and instrument configurations which illuminate the concepts and principles behind the types of optical microscopy that you can do in the Advanced Cellular and Tissue Microscopy.
Olympus, Microscope Configurations
ThermoFisher, Molecular Probes School of Fluorescence
We recommend you follow the instructions from the manufactures for the immunofluorescence staining procedure as well as for regents, supplies and procedures. However, please feel free to contact us with any questions you may have on imaging and sample preparations.