Atherosclerosis and Lipoprotein Research Lab
Henry J. Pownall, PhD
Professor of Bioenergetics
Weill Cornell Medical College
The Laboratory of Atherosclerosis and Lipoprotein Research seeks to identify the mechanisms underlying atherogenesis and how to prevent it. Foremost among these mechanisms is reverse cholesterol transport (RCT), i.e., transfer of cholesterol from the arterial wall and other peripheral tissues to the liver for disposal. The major RCT mediator is high density lipoproteins (HDL); thus the lab’s focus is HDL biogenesis, the mechanisms by which distinct HDL subspecies are formed, and the role of specific amino acids within the major HDL proteins, apolipoprotein AI, in HDL formation. Lab staff also discovered an alternative RCT pathway and plan to further validate this pathway and determine whether it is impaired in patients with atherosclerosis. Testing a newly-identified cholesterol-lowering bacterial protein will aid in the design of new anti-atherogenic therapies that enhance RCT. On the clinical side, the lab participates in a large trial (LookAhead) of the effects of weight loss among obese, diabetic patients.
Henry J. Pownall, PhD – Scientist, Principal Investigator
His research is multidisciplinary, with extensive collaboration with structural biologists, endocrinologists, and x-ray crystallographers. Structural biologists provide expertise in lipid and lipoprotein structure by electron cryo microscopy. Endocrinologists have synergistic interests in the lipid disorders found in HIV-positive patients on highly active anti retroviral therapies. X-ray crystallographers help discern the total structure of Streptococcal serum opacity factor (SOF) for determination of structure function relationships.
Dr. Pownall maintains an active academic program. He is a member of three graduate programs at Baylor College of Medicine (Structural Computational Biology and Molecular Biophysics, Cell and Molecular Biology, and Cardiovascular Sciences) in which he teaches classes and serves on graduate advisory and qualifying exams committees. He is also active in community education, giving lectures on molecular ethics to local groups including several churches, home owner associations and garden clubs.
Baiba Gillard, PhD – Research Scientist
Baiba Gillard, PhD, received a doctorate in physical organic chemistry from Washington University in St. Louis, Missouri. She conducted postdoctoral training in biochemistry at Baylor College of Medicine and in biomedical research at UCLA. Gillard joined the faculty of the Department of Medicine at Baylor College of Medicine to work with Donald Marcus, MD, in glycobiology. Since 2002, she has worked with Henry Pownall, PhD, gaining research expertise in lipid and lipoprotein metabolism and atherosclerosis. In the Pownall laboratory, Gillard is in charge of cell biology of numerous athero-, diabetes-, and lipid-relevant cell lines, and lipid analysis. She is currently a research scientist at HMRI, and a faculty member of Weill Cornell Medical College.
- Gillard, BK, PJ Rodriguez, DW Fields, JL Raya, WR Lagor, C Rosales, HS Courtney, AM Gotto Jr and HJ Pownall. Streptococcal Serum Opacity Factor promotes Cholesterol Ester Metabolism and Bile Acid Secretion In Vitro and In Vivo. Biochim Biophys Acta 1861:196-204, 2016. PMID: 26709142
- Gillard, BK, GR Bassett, AM Gotto, Jr, C Rosales and HJ Pownall HJ. Scavenger receptor B1 (SR-B1) profoundly excludes high density lipoprotein (HDL) apolipoprotein AII as it nibbles HDL-cholesteryl ester. J Biol Chem 292:8864-8873, 2017. PMID: 28373285.
- Xu B, BK Gillard, AM Gotto, Jr., C Rosales and HJ Pownall. ABCA1-Derived Nascent High Density Lipoprotein-Apolipoprotein AI and Lipids Metabolically Segregate. Arterioscler Thromb Vasc Biol 37:2260-2270, 2017. PMID: 29074589.