Houston Methodist. Leading Medicine.
Houston Methodist. Leading Medicine

Zheng-zheng Zhi

Zheng-Zheng Shi, M.D., Ph.D.


Zheng-Zheng Shi, M.D.

Zheng-Zheng Shi, M.D., Ph.D.

Assistant Member
Department of Translational Imaging
The Methodist Hospital Research Institute
Research Scientist
Department of Radiology 
The Methodist Hospital
Assistant Research Professor
Weill Medical College of Cornell University

E-mail: zshi@houstonmethodist.org
Phone: 713-441-9601


M.S.   Zheijang Medical University, Hangzhou, China (Pathophysiology)
M.D.  Zheijang Medical University, Hangzhou, China
Ph.D. Zhejiang University, Hangzhou, China (Oncology)

Postdoctoral Training

Postdoctoral fellow, Department of Pathology, Baylor College of Medicine, Houston, TX



After completing his postdoctoral training, Dr. Shi became an Instructor (1997) and then Assistant Professor (1999) at the Baylor College of Medicine in Houston, Texas. His research focused on the molecular biology of glutathione (GSH), a dominant intracellular antioxidant. Dr. Shi and his colleagues first identified mutations in GSH synthetase as the cause of the disease 5–oxoprolinuria, a rare but serious human genetic disorder. Using knockout technology, they first proved that GSH is essential for mammalian development by dispensing it in cultured mammalian cells. In 2000, Dr. Shi joined Lexicon Genetics (The Woodlands, TX) where he established and oversaw a large-scale phenotypic analysis of knockout mice. As a Senior Scientific Group Leader, he also developed the use of small animal imaging systems including magnetic resonance imaging (MRI) and micro–computed tomography (micro–CT) for phenotypic analysis and pre-clinical testing. He joined the Methodist Hospital Research Institute in 2007 as Chief of Small Animal Imaging and a Research Scientist in the Department of Radiology.

Description of Research

The long-term goals of Dr. Shi´s research are: (i) to discover novel cellular proteins/pathways that can serve as therapeutic targets for human diseases including cancer, or as targets for molecular imaging; and (ii) to support the development of novel molecular imaging agents/methods by providing expertise and core services in molecular biology and small animal imaging. His laboratory has recently discovered a novel Obg–like ATPase (OLA1) which functions as a regulator of oxidative stress response and many other cellular functions through translational and post-translational pathways. His group is currently focusing on demonstration of in vivo physiological functions of OLA1 and validation of OLA1 as an anti-cancer or anti-metastasis target.

Major Areas of Research

Molecular imaging, oxidative stress, post–translational regulation, tumor metastasis

Recent Publications

Ghosh P, Zhang J, Shi ZZ, Li K. Synthesis and evaluation of an imidazole derivative-fluorescein conjugate. Bioorg Med Chem. 2013 Apr 15;21(8):2418-25. PMID: 23477941

Mao RF, Rubio V, Chen H, Bai L, Mansour OC, Shi ZZ. OLA1 protects cells in heat shock by stabilizing HSP70. Cell Death Dis. 2013 Feb 14;4:e491. PMID: 23412384

Sun C, Shi ZZ, Zhou X, Chen L, Zhao XM. Prediction of S-glutathionylation sites based on protein sequences. PLoS One. 2013;8(2):e55512. PMID: 23418443

Wang X, Minze LJ, Shi ZZ. Functional imaging of brown fat in mice with 18F-FDG micro-PET/CT. J Vis Exp. 2012 Nov 23;(69). PMID: 23207798

Van Sligtenhorst I, Ding ZM, Shi ZZ, Read RW, Hansen G, Vogel P. Cardiomyopathy in a-Kinase 3 (ALPK3)-Deficient Mice. Vet Pathol. 2012 Jan;49(1):131-41. PMID:21441111

Kim YS, Rubio V, Qi J, Xia R, Shi ZZ, Peterson L, Tung CH, O'Neill BE. Cancer treatment using an optically inert Rose Bengal derivative combined with pulsed focused ultrasound. J Control Release. 2011 Dec 20;156(3):315-22. PMID: 21871506 

Winkler A, Njålsson R, Carlsson K, Elgadi A, Rozell B, Abraham L, Ercal N, Shi ZZ, Lieberman MW, Larsson A, Norgren S. Glutathione is essential for early embryogenesis--analysis of a glutathione synthetase knockout mouse. Biochem Biophys Res Commun. 2011 Aug 19;412(1):121-6. PMID: 21802407 

Wen J, Feng Y, Bjorklund CC, Wang M, Orlowski RZ, Shi ZZ, Liao B, O'Hare J, Zu Y, Schally AV, Chang CC. Luteinizing Hormone-Releasing Hormone (LHRH)-I antagonist cetrorelix inhibits myeloma cell growth in vitro and in vivo. Mol Cancer Ther. 2011 Jan;10(1):148-58. PMID: 21062912

Rubio V, Zhang J, Valverde M, Rojas E, Shi ZZ. Essential role of Nrf2 in protection against hydroquinone- and benzoquinone-induced cytotoxicity. Toxicol In Vitro. 2011 Mar;25(2):521-9. PMID: 21059386

Feng Y, Wen J, Mike P, Choi DS, Eshoa C, Shi ZZ, Zu Y, Chang CC. Bone marrow stromal cells from myeloma patients support the growth of myeloma stem cells. Stem Cells Dev. 2010 Sep;19(9):1289-96. PMID: 20121456

Li F, Liu J, Jas GS, Zhang J, Qin G, Xing J, Cotes C, Zhao H, Wang X, Diaz LA, Shi ZZ, Lee DY, Li KC, Li Z. Synthesis and evaluation of a near-infrared fluorescent non-peptidic bivalent integrin alpha(v)beta(3) antagonist for cancer imaging. Bioconjug Chem. 2010 Feb 17;21(2):270-8. PMID: 20102181

Zhang J, Rubio V, Lieberman MW, Shi ZZ. OLA1, an Obg-like ATPase, suppresses antioxidant response via nontranscriptional mechanisms. Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15356-61. PMID: 19706404