Richard Federley, PhD, has served as a chemistry instructor and invited lecturer. Serving as a research investigator, he has special interests in DNA replication, enzyme kinetics, site specific DNA modification and purification, mass spectrometry, methods development, and surface plasmon resonance. Obtaining his Bachelor of Science in Biology and a Ph.D in Chemistry, he has authored a number of published works and was instrumental in developing efficient HPLC protocols for the purification of various oligonucleotides along with a simple and rapid method for the analysis of these oligonucleotides using MALDI-TOF mass spectrometry - which have translated into successful procedures now routinely used.
- Deduced a novel pathway in the kinetic mechanism of the DNA polymerase Klenow fragment, which accurately predicts the influence of various incoming nucleotides on its binding behavior.
- Discovered that a G:G-benzo[a]pyrene mismatch forms a unique structure causing Klenow fragment to remain bound more tightly than compared to other benzo[a]pyrene-modified substrates, possibly further contributing to carcinogenesis.
- Performed large scale expression and purification of thiaminase (type 1) from E. coli.
- In field collection of alewives for later testing for thiaminase activity by future students.
- Aided in the development of a novel fluorescence based assay for the direct detection of thiaminase activity.
- Completed testing of the novel fluorescence assay and performed direct comparisons to other more labor intensive assays.
Ph.D. in Chemistry - Wayne State University, Detroit MI
B.S. in Biology - Lake Superior State University, Sault Ste. Marie MI
Awards and Grants
- Graduate School Citation for Excellence in Teaching - Wayne State University
- Dean’s Honor’s List - Lake Superior State University
- Undergraduate Research Grant - Lake Superior State University
- Board of Trustees Ontario Academic Scholarship - Lake Superior State University
- Federley RG, Romano LJ. DNA polymerase: structural homology, conformational dynamics, and the effects of carcinogenic DNA adducts. J. Nucleic Acids. 2010, 22;2010. pii: 457176.
- O’Toole M., Federley RG, Werner M. Thiaminase: progress toward a novel fluorescent microplate assay. Biochemistry, 2002 (abstract).
- Federley RG, Romano LJ. DNA polymerase actively rejects mispairing dNTPs during DNA synthesis: evidence for a new cycle in the DNA polymerase mechanism (manuscript complete)
- Federley RG, Romano LJ. A(-) - trans benzo[a]pyrene labelled guanine-guanine mismatch at the primer terminus forms a distinct complex when bound to Klenow Fragment (manuscript completed)
- Gill JP, Federley RG, Romano LJ. 2-aminofluorene lesion bypass on the NarI sequence by Escherichia coli DNA polymerase I Klenow Fragment (manuscript completed).