Houston Methodist. Leading Medicine.
Houston Methodist. Leading Medicine

Resources List

Resources List

 

Microscopes

1. Fluo ViewTM 1000 Confocal Microscope (R6-411)

Five lasers: 405 Laser Diode; 458, 488, 515 mArgon; 543 HeNe; 633 HeNe; Near-infrared LD748

The Olympus FV1000 is a next-generation imaging system designed for high-resolution, confocal observation of both fixed and living cells. The FV1000 offers advances in confocal system performance while providing the speed and sensitivity required for live cell imaging with minimal risk of damage to living specimens. In addition, the FV1000 offers a revolutionary synchronized laser scanning system called the SIM Scanner. While one laser stimulates, the second laser simultaneously provides high-resolution imaging. This coordination of laser stimulation and imaging makes the FV1000 an ideal choice for FRAP, FLIP and photo-activation.

2. Live Cell Confocal Image System (R6-411)

The system consists of IX81 automated inverted microscope, SRV CCD Digital Camera and CO2/humidity/temperature control incubator. It especially afford good conditions for live cell phase contrast, DIC, various fluorescent imaging. The filters sets are for DAPI, Hoechst, and AMCA excitation; for FITC/EGFP/BODIPY/FUO3DIO excitation, for TEXAS RED and for CY5. The objectives are 4X, 10X, 20X, 40X, 40X oil, 60Xoil and 100X oil.

3. Live Cell Spinning disk Confocal Image System (R6-411)

http://www.olympusamerica.com/seg_section/product.asp?product=1009

  • Disk control is fully motorized allowing a computer to easily engage the disk into the light path and select wavelengths via the included filter changer.
  • Image formation is obtained from a EM-CCD camera that allows full frame images to be acquired at up to 15 frames per second. The DSU is excellent for live-cell applications where speed of acquisition and minimal phototoxicity is paramount.
  • Fully motorized disk operation and wavelength selection allows computer control and easy switching between confocal and wide-field techniques.

4. INCUCYTETM Live-Cell Imaging System (R6-411)

IncuCyte allows you to place a microscope inside your incubator, taking advantage of an asset you already own as well as eliminating any potential problems that may result from disturbing cells during the observation process. This IncuCyte approach makes long-term kinetic imaging convenient and affordable. It provides a more efficient and thorough set of solutions for long-term, live-cell imaging. The IncuCyte is designed with increased productivity in mind supporting hundreds of standard varieties of cell culture vessels, including 96- and 384-well plates, T-flasks, dishes, and micro-slides. With three configurable trays, you can mix and match multiple vessels and even monitor different experiments running concurrently.

5. CRI Nuance Multispectral Imaging System (R6-412)

http://www.cri-inc.com/products/nuance.asp?gclid=CICW3eS4rZoCFSMgDQodm11vbw

Nuance has two main advantages over confocal multispectral systems. First, it is able to do bright field imaging of chromogenic molecular markers while the confocal imaging systems are restricted to fluorescence-based applications. Secondly, in fluorescence microscopy, in samples which are auto-fluorescent, Nuance is superior to most laser-scanning multispectral confocal systems at separating tissue auto-fluorescence from signals of interest. This is partly because of Nuance’s light-efficient CCD-based imaging system, which is excellent for shot-noise limited samples and partly because of Nuance’s algorithms for determining the pure spectral signatures of fluorophores from tissue sections with auto-fluorescence.

6. Total Internal Reflection Fluorescence Microscopy (R6-412)

http://www.olympusmicro.com/primer/techniques/fluorescence/tirf/tirfhome.html

Total internal reflection microscopy (TIRFM) is an optical technique used to observe single molecule fluorescence. Today, the technique is gaining popularity with cell biologists and neuroscientists to employ it to observe cell membrane fluorescence, in part because new membrane-specific dyes have been developed. Two laser lines are setup for TIFF imaging (458, 488, 515 mArgon and 543 HeNe). Major advantages of TIRF: 1) discreet structures and single molecules can be visualized without interference from ubiquitous fluorophores. 2): high signal-to-noise ratios. The environmental is fully controlled by the Tokai Hit Stage Top incubator which provides accurate control of the temperature, CO2 and humidity for long-term time-lapse imaging of living cells and tissues.

7. Regular Inverted BF&Fluorescent Microscope (R6-412)

The IX81 begins with a unique two-tiered, V-shaped optical design that provides tremendous versatility without frame modifications and bright images with minimal reflections. Optional 6-position fluorescence filter turret accepts a unique analyzer holder for switching between DIC/polarized light and fluorescence observations. With its built-in motorized Z-axis drive, 6-position nosepiece and light path selector, the IX81 is ready for multi-wavelength, advanced fluorescence and de-convolution techniques.

8. Regular Upright BF&Fluorescent Microscope (R6-411)

The BX61 Completely modular frame and optical design provides a flexible, fast, reliable and precise upright BF/fluorescent imaging system. It is recently upgraded with reflecting imaging.

9. Nikon A1 Confocal Imaging System (R9-317)

  • Hybrid Confocal Scan Head: Unique hybrid confocal scan head, enhanced the optical efficiency and minimized light loss.
  • Ultra High-Speed Imaging at 420 fps: A resonant scanner with a resonance frequency of 7.8kHz that allows high-speed imaging at 420 fps (512 x 32 pixels).
  • Enhanced Spectral Imaging: Fast spectral imaging at 16 fps (512 x 64 pixels) is possible and real-Time Spectral Unmixing
  • Increased Light Detection Efficiency: Average transmittance of 98% and contribute to a 30% increase in fluorescence detection efficiency.

10. Laser Capture Micro-dissection (LCM, Arcturus) (R6-412)

This Laser Capture Micro-dissection (LCM) method is extraordinarily gentle and ideal for micro-dissection of single cells or small numbers of cells. The ArcturusXT™ micro-dissection instruments each combine LCM and UV laser cutting for ultimate micro-dissection flexibility.

These instruments remove the guesswork from the micro-dissection process by allowing researchers to maintain custody of the sample throughout the experiment, ensuring that only the desired material has been collected.

11. ImageStreamX---High resolution microscopy in flow (R6-412)

The ImageStreamX combines the high-speed capture of high-resolution imagery with detailed quantitation to create a statistically robust microscopy platform for a wide range of cell analysis applications.

ImageStreamX produces up to 12 high resolution images of each cell directly in flow, at rates exceeding 1,000 cells per second, and with the fluorescence sensitivity of the best conventional flow cytometers. These breakthrough capabilities allow you to quantitate cellular morphology and the intensity and location of fluorescent probes on, in, or between cells, even in rare sub-populations and highly heterogeneous samples.

  • Image cells directly in suspension with the resolution of a 60X microscope and the fluorescence sensitivity of the best flow cytometers
  • Analyze highly heterogeneous samples and rare cell sub-populations at speeds exceeding 1,000 cells per second
  • Perform phenotypic and functional studies at the same time using up to five lasers and 12 images per cell
  • Quantitate virtually anything you can see using the IDEAS® software package’s numerous pre-defined fluorescence and morphologic parameters



Peripheral Equipment

  • Live cell microscopy environmental control system.
  • Tissue culture incubator (37°C w/ 5% CO2) for short-term storage.
  • Cryosection machine.
  • Cyto centrifugation .
  • -80°C freezer.
  • -20°C freezer.

Workstation

  • Dell Precision 390 workstation

Software

  • Olympus DP-BSW image capture and control software
  • Olympus LSM Software package Intelligent Imaging Innovations’ SlideBook Software package for 2D and 3D imaging on PC computer platforms.
For customized software development of specialized bioassays, please contact Dr. Stephen Wong, stwong@houstonmethodist.org