|High content screening and analysis of dendrite spine morphology
Acquisition and quantitative analysis of high resolution images of dendritic spines are challenging tasks but are necessary for the study of animal models of neurological and psychiatric diseases. Currently available methods for automated dendritic spine detection are, for the most part, customized for 2D image slices, not volumetric 3D images. By using the NeuronIQ (Neuron Image Quantitator) project, we intend to develop a fully automated method for the detection and segmentation of dendritic spines from 3D confocal microscopy images; neuron types include normal neurons, neurodegenerative neurons, and medium-sized spiny neurons (MSNs). Automated detection is critical for the development of new 3D neuronal assays which can be used for the screening of drugs and studies of their therapeutic effects.