|Faculty: Lidong Qin, Ph.D.|
Problem 1: Although currently available biotechnology has provided us with a greater understanding of the molecular events in cancer, integrated microfluidic chips provide unique methodologies that can recapitulate the spatial and temporal control of cell proliferation and cell-cell/matrix communication by combining surfaces that mimic the complex biochemistries and geometries of the extracellular matrix.
Approach 1: This research focuses on developing integrated proteomic microchips to analyze cell heterogeneity using state-of-the-art bioinformatics tools and identifying metastatic signatures. The research aims are to deliver brand new technologies and methodologies capable of identifying tumor-initiating cells, discovering potential biomarkers for clinical diagnosis and targeted therapy, and identifying cancer patients with metastatic propensity.
|Typical Microfluidics Device. (A) An autocad drawing to show our design of single cell barcode chip (SCBC). This schematic picture presents 100 horizontal channels X 36 vertical valves and creates 3600 microchambers for the single-cell experiment. Chamber number could expand to 300 X 50 = 15,000 for a PDMS device bonding to a 75 mm X 50 mm glass substrate. (B) A typical scanned protein barcode image zoomed from a portion of the SCBC device. The protein expression level from single cell or a few cell chambers is measured by fluorescent intensity shown as red bars in between green bar pairs. Yellow numbers represent cell number counted from the experiment, resulting in the barcode image.|
Problem 2: Inspired by a global need for better diagnosis and treatment strategies, this research focuses on point-of-care (POC) challenges to ultimately design a flexible multi-platform microfluidic device for the rapid and direct characterization of rare, disease-related cells for individualized patient care.
|Design of enhanced Metastasis Assessing Chip (eMAC) for rapid anti-metastasis drug tests. The design contains an engineered tissue pad made of MatrigelTM and stroma cells. It also contains cancer cell culture chambers, a drug test, and a secreted protein expression test.|
Approach 2: We intend to globally revolutionize disease diagnosis and treatment strategies by:
Current & Previous Grant Support: