We will characterize and assess every change in every gene in the entire human genome that is associated with and may cause each patient’s cancer. Similar analyses will occur for changes in proteins associated with and possible causing the cancer in question.
The field of proteomics strives to understand how proteins—the molecular entities that make up the major structural and functional components of cells and that drive physiological processes within all cells and tissues—relate to each other, and how that relation changes under altered physiological conditions, such as disease. Proteomics is thus the scientific discipline that strives to understand disease processes in terms of measuring the abundance, tissue localization, structure, function, modifications and molecular interactions proteins undergo as they regulate physiological processes, and how they may be altered during pathophysiology.
This is accomplished in many different ways under many different research scenarios, but it often employs workflow strategies and technology platforms that require various suitable protein isolation techniques followed by protein/peptide-level separation technologies and mass spectrometry to help identify and quantify proteins isolated from biological samples and tissues.
The Peak Center has access to this resource, which is equipped and staffed to facilitate such translational biomedical research aimed at understanding the mechanisms and regulation of biological processes controlled at the protein level via the measurement and molecular characterization of proteins isolated from cells and tissues. This is accomplished by various techniques, including mass spectrometry-based techniques, which is the primary technological platform upon which the existing RI Proteomics Core is structured.
The Proteomics Lab is set up to offer a wide array of services for typical proteomic analysis needs. These services and proteomic workflows are primarily set up around liquid chromatography-mass spectrometry (LC/MS) instrumentation platforms. These, along with associated sophisticated data analysis software and computational platforms and database search engines, form the basis of the Lab’s existing technology offerings. The Lab provides a full complement of scientific support in addition to its analytical instrumentation service capabilities—meaning its scientists are involved with the investigator from the experimental design phase of a study all the way through to data analysis and interpretation at all levels of proteomic research,