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Henry J. Pownall, PhD

Professor of Bioenergetics, Institute for Academic Medicine
Full Member, Research Institute
Houston Methodist
Weill Cornell Medical College


hjpownall@houstonmethodist.org
Biography

Dr. Pownall’s doctoral training was in physical chemistry with postdoctoral fellowships in molecular spectroscopy at the University of Houston, and biochemistry at Baylor College of Medicine with an emphasis on lipid metabolism. Over time he moved his studies from molecules to in vivo models, studying biophysics, peptide design, lipid synthesis and enzymology, and cell and molecular biology. His work is centered on high density lipoprotein therapeutics, and energy metabolism as it relates to obesity-linked diabetes and human lipid metabolism. A major goal of his research is to determine how alcohol ingestion contributes to enhanced postprandial lipemia, attendant pancreatitis, or alcohol-induced reduction of cardiovascular disease, an effect mediated by increased HDL-cholesterol.

His research is multidisciplinary, with extensive collaboration with structural biologists, endocrinologists, and x-ray crystallographers. Structural biologists provide expertise in lipid and lipoprotein structure by electron cryo microscopy. Endocrinologists have synergistic interests in the lipid disorders found in HIV-positive patients on highly active anti retroviral therapies. X-ray crystallographers help discern the total structure of Streptococcal serum opacity factor (SOF) for determination of structure function relationships.

Dr. Pownall maintains an active academic program. He is a member of three graduate programs at Baylor College of Medicine (Structural Computational Biology and Molecular Biophysics, Cell and Molecular Biology, and Cardiovascular Sciences) in which he teaches classes and serves on graduate advisory and qualifying exams committees. He is also active in community education, giving lectures on molecular ethics to local groups including several churches, home owner associations and garden clubs.

Description of Research

Dr. Pownall’s studies encompass multiple aspects of lipid metabolism, particularly HDL structure, properties and function in the context of obesity-linked diabetes and atherogenesis. His research goals are as follows:

-To determine if Streptococcal serum opacity factor (SOF) can enhance cholesterol disposal and reverse atherosclerosis
-To obtain a high resolution X-ray structure of SOF for the identification of active sites and design of SOF analogs
-To compare the energy storage potential of central and femoral gluteal depots, as they relate to obesity-linked diabetes
-To assess the effects of acetate, as an alcohol metabolite and in the food supply, on HDL levels and the onset of pancreatitis

Areas Of Expertise

Physical chemistry Obesity-linked diabetes Cholesterol transport Atherogenesis Fluorescence
Education & Training

Postdoctoral Fellowship , Baylor College of Medicine
PhD , Northeastern University
Postdoctoral Fellowship , The University of Houston
MS , Wilkes College, Wilkes-Barre, PA
Publications

ABCA1-Derived Nascent High-Density Lipoprotein-Apolipoprotein AI and Lipids Metabolically Segregate
Xu, B, Gillard, BK, Gotto, AM, Rosales, C & Pownall, HJ 2017, Arteriosclerosis, thrombosis, and vascular biology, vol 37, no. 12, pp. 2260-2270. DOI: 10.1161/ATVBAHA.117.310290

The Effect of Intentional Weight Loss on Fracture Risk in Persons With Diabetes: Results From the Look AHEAD Randomized Clinical Trial
Johnson, KC, Bray, GA, Cheskin, LJ, Clark, JM, Egan, CM, Foreyt, JP, Garcia, KR, Glasser, S, Greenway, FL, Gregg, EW, Hazuda, HP, Hergenroeder, A, Hill, JO, Horton, ES, Jakicic, JM, Jeffery, RW, Kahn, SE, Knowler, WC, Lewis, CE, Miller, M, Montez, MG, Nathan, DM, Patricio, JL, Peters, AL, Pi-Sunyer, X, Pownall, HJ, Reboussin, D, Redmon, JB, Steinberg, H, Wadden, TA, Wagenknecht, LE, Wing, RR, Womack, CR, Yanovski, SZ, Zhang, P, Schwartz, AV & Look AHEAD Study Group 2017, Journal of Bone and Mineral Research, vol 32, no. 11, pp. 2278-2287. DOI: 10.1002/jbmr.3214

Physical Function Following a Long-Term Lifestyle Intervention among Middle Aged and Older Adults with Type 2 Diabetes: the Look AHEAD Study
Houston, DK, Neiberg, RH, Miller, ME, Hill, JO, Jakicic, JM, Johnson, KC, Gregg, EW, Hubbard, VS, Pi-Sunyer, X, Rejeski, WJ, Wing, RR, Bantle, JP, Beale, E, Berkowitz, RI, Cassidy-Begay, M, Clark, JM, Coday, M, Delahanty, LM, Dutton, G, Egan, C, Foreyt, JP, Greenway, FL, Hazuda, HP, Hergenroeder, A, Horton, ES, Jeffery, RW, Kahn, SE, Kure, A, Knowler, WC, Lewis, CE, Martin, CK, Michaels, S, Montez, MG, Nathan, DM, Patricio, J, Peters, A, Pownall, H, Regensteiner, J, Steinburg, H, Wadden, TA, White, K, Yanovski, SZ, Zhang, P & Kritchevsky, SB 2017, Journals of Gerontology - Series A Biological Sciences and Medical Sciences. DOI: 10.1093/gerona/glx204

Scavenger receptor B1 (SR-B1) profoundly excludes high density lipoprotein (HDL) apolipoprotein AII as it nibbles HDL-cholesteryl ester
Gillard, BK, Bassett, GR, Gotto, AM, Rosales, C & Pownall, HJ 2017, Journal of Biological Chemistry, vol 292, no. 21, pp. 8864-8873. DOI: 10.1074/jbc.M117.781963

Scavenger receptor B1 (SR-B1) profoundly excludes high density lipoprotein (HDL) apolipoprotein AII as it nibbles HDL-cholesteryl ester
Gillard, BK, Bassett, GR, Gotto, AM, Rosales, C & Pownall, HJ 2017, Journal of Biological Chemistry, vol 292, no. 21, pp. 8864-8873. DOI: 10.1074/jbc.M117.781963

Effect of a long-term intensive lifestyle intervention on prevalence of cognitive impairment
Espeland, MA, Luchsinger, JA, Baker, LD, Neiberg, RH, Kahn, SE, Arnold, SE, Wing, RR, Blackburn, GL, Bray, GA, Evans, M, Hazuda, HP, Jeffery, RW, Wilson, VM, Clark, JM, Coday, M, Demos-McDermott, K, Foreyt, JP, Greenway, FL, Hill, JO, Horton, ES, Jakicic, JM, Johnson, KC, Knowler, WC, Lewis, CE, Nathan, DM, Peters, AL, Pi-Sunyer, X, Pownall, H, Wadden, TA, Rapp, SR & Look AHEAD Study Group 2017, Neurology. DOI: 10.1212/WNL.0000000000003955

Cellular ABCA1-Derived Nascent HDL-Cholesterol Transfers to the LDLR Pathway.
Xu, B, Gillard, BK, Gotto, A, Rosales, C & Pownall, HJ 2017, European Atherosclerosis Society, Prague, Czech Republic, 4/23/17 - 4/26/17, .

The HDL receptor, SR-B1, forms remnant HDL by a nibbling mechanism and releases apo AI.
Gillard, BK, Bassett, GR, Rosales, C, Gotto, A & Pownall, HJ 2017, European Atherosclerosis Society, Prague, Czech Republic, 4/23/17 - 4/26/17, .

Structural Stability of Streptococcal Serum Opacity Factor.
Rosales, C, Yelamanchili, D, Gillard, BK, Gotto, A & Pownall, HJ 2017, European Atherosclerosis Society, Prague, Czech Republic, 4/23/17 - 4/26/17, .

Structural Stability of Streptococcal Serum Opacity Factor
Yelamanchili, D, Gillard, BK, Gotto, AM, Pownall, HJ & Rosales, C 2017, Protein Journal, pp. 1-6. DOI: 10.1007/s10930-017-9711-4

Metabolic Segregation of Nascent HDL-Lipid and Protein.
Xu, B, Gillard, BK, Gotto, A, Rosales, C & Pownall, HJ 2017, BCM CVRI Symposium, Houston, United States, 4/4/17 - 4/4/17, .

Scavenger receptor B1 (SR-B1) profoundly excludes high density lipoprotein (HDL) apolipoprotein AII as it nibbles HDL-cholesteryl ester
Gillard, BK, Bassett, GR, Gotto, AM, Rosales, C & Pownall, HJ 2017, The Journal of biological chemistry, vol 292, no. 21, pp. 8864-8873. DOI: 10.1074/jbc.M117.781963

The HDL Receptor, SR-B1 Forms Remnant HDL by a Nibbling Mechanism and Releases Apo AI
Pownall, HJ 2017, European Atherosclerosis Society, Prague, Czech Republic, 4/23/17 - 4/26/17, .

AIBP Limits Angiogenesis Thorough ?-Secretase-Mediated Upregulation of Notch Signaling
Mao, R, Meng, S, Gu, Q, Araujo-Gutierrez, R, Kumar, S, Yan, Q, Almazan, F, Youker, KA, Fu, Y, Pownall, HJ, Cooke, JP, Miller, YI & Fang, L 2017, Circulation Research. DOI: 10.1161/CIRCRESAHA.116.309754

Somatic genome editing with CRISPR/Cas9 generates and corrects a metabolic disease
Jarrett, KE, Lee, CM, Yeh, Y-H, Hsu, RH, Gupta, R, Zhang, M, Rodriguez, PJ, Lee, CS, Gillard, BK, Bissig, K-D, Pownall, HJ, Martin, JF, Bao, G & Lagor, WR 2017, Scientific Reports, vol 7, pp. 44624. DOI: 10.1038/srep44624

Association of the magnitude of weight loss and changes in physical fitness with long-term cardiovascular disease outcomes in overweight or obese people with type 2 diabetes: a post-hoc analysis of the Look AHEAD randomised clinical trial
Gregg, E, Jakicic, J, Blackburn, G, Bloomquist, P, Bray, GA, Clark, J, Coday, M, Curtis, J, Egan, C, Evans, M, Foreyt, JP, Foster, GD, Hazuda, HP, Hill, J, Horton, E, Hubbard, VS, Jeffery, RW, Johnson, KC, Kitabchi, AE, Knowler, WC, Kriska, A, Lang, W, Lewis, CE, Montez, M, Nathan, DM, Neiberg, RH, Patricio, J, Peters, AL, Pi-Sunyer, X, Pownall, H, Redmon, B, Regensteiner, JG, Rejeski, J, Ribisl, P, Safford, M, Stewart, K, Trence, DL, Wadden, TA, Wing, RR, Yanovski, SZ & Look AHEAD Research Group 2016, The Lancet Diabetes and Endocrinology, vol 4, no. 11, pp. 913-921. DOI: 10.1016/S2213-8587(16)30162-0

Acylation of lysine residues in human plasma high density lipoprotein increases stability and plasma clearance in vivo
Yang, Y, Rosales, C, Gillard, BK, Gotto, AM & Pownall, HJ 2016, Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, vol 1861, no. 11, pp. 1787-1795. DOI: 10.1016/j.bbalip.2016.08.017

Neo High-Density Lipoprotein Produced by the Streptococcal Serum Opacity Factor Activity against Human High-Density Lipoproteins Is Hepatically Removed via Dual Mechanisms
Rodriguez, PJ, Gillard, BK, Barosh, R, Gotto, AM, Rosales, C & Pownall, HJ 2016, Biochemistry, vol 55, no. 41, pp. 5845-5853. DOI: 10.1021/acs.biochem.6b00946

Native and Reconstituted Plasma Lipoproteins in Nanomedicine: Physicochemical Determinants of Nanoparticle Structure, Stability, and Metabolism
Pownall, HJ , Rosales, C , Gillard, BK & Ferrari, M 2016, Houston Methodist DeBakey Cardiovascular Journal, vol 12, no. 3, pp. 146-150. DOI: 10.14797/mdcj-12-3-146

Changes in regional body composition over 8 years in a randomized lifestyle trial: The look AHEAD study
Pownall, HJ, Schwartz, AV, Bray, GA, Berkowitz, RI, Lewis, CE, Boyko, EJ, Jakicic, JM, Chen, H, Heshka, S, Gregg, EW & Johnson, KC 2016, Obesity, vol 24, no. 9, pp. 1899-1905. DOI: 10.1002/oby.21577