Houston Methodist. Leading Medicine.
Houston Methodist. Leading Medicine

James Mancuso

James J. Mancuso, Ph.D.


James J. Mancuso, Ph.D.

James J. Mancuso, Ph.D.

Instructor and Research Associate
Houston Methodist Research Institute
NeuroScience Imaging & Electrophysiology Lab
Department of Systems Medicine and Bioengineering

E-mail: mancusojj@houstonmethodist.org
Phone: 713-441-6365


B.S. Louisiana State University (Biochemistry and Microbiology)
Ph.D. Baylor College of Medicine (Biochemistry)

Postdoctoral Training

Postdoctoral Fellow, Laboratory of Synaptic Circuitry, Duke-NUS Singapore



During his graduate work in Ted Wensel’s lab at Baylor College of Medicine Dr. Mancuso applied protein biochemistry, optical imaging, and patch clamp electrophysiology to study neuronal signaling pathways associated with Parkinson’s disease, schizophrenia, and drug addiction. Upon completion of his Ph.D. studies, Dr. Mancuso did a two year postdoctoral fellowship in George Augustine’s lab at Duke-NUS in Singapore studying neuronal physiology and network connectivity using single and two-photon activation of optogenetic effectors and traditional electrophysiology. In September 2011 he joined the Chao Center for BRAIN and the Department of Systems Medicine and Bioinformatics in the Neuroscience Imaging and Electrophysiology lab. He is currently collaborating with a number of other researchers to find effective treatments for a variety of devastating neurodegenerative disorders.

Description of Research

Dr. Mancuso’s research program is exploring the way that neurodegenerative diseases cause anatomical and physiological changes in the connections between specific types of cells in the brain in order to identify more precise targets for treatment. Current work is utilizing intravital two-photon imaging of brain function, optogenetics, and patch clamp electrophysiology to tackle neurodegeneration on a systems neuroscience level with single-cell resolution.

Major Areas of Research

Neurodegeneration, two-photon, optogenetics, intravital microscopy, synapse

Recent Publications

Chen Y, Mancuso J, Zhao Z, Li X, Cheng J, Roman G, Wong ST. Vasodilation by in vivo activation of astrocyte endfeet via two-photon calcium uncaging as a strategy to prevent brain ischemia. J Biomed Opt. 2013 Dec 1;18(12):126012. PMID: 24343443

Mancuso JJ, Chen Y, Li X, Xue Z, Wong ST. Methods of Dendritic Spine Detection: from Golgi to High Resolution Optical Imaging. Neuroscience. 2013 Oct 22;251:129-40. PMID: 22522468

Mancuso JJ, Kim J, Lee S, Tsuda S, Chow NB, Augustine GJ. Optogenetic probing of functional brain circuitry. Exp Physiol. 2011 Jan;96(1):26-33. PMID: 21056968

Mancuso JJ, Qian Y, Long C, Wu GY, Wensel TG. Distribution of RGS9-2 in neurons of the mouse striatum. J Neurochem. 2010 Feb;112(3):651-61. PMID:19912469

Mancuso JJ, Larson AM, Wensel TG, Saggau P. Multiphoton adaptation of a commercial low-cost confocal microscope for live tissue imaging. J Biomed Opt. 2009 May-Jun;14(3):034048. PMID: 19566340