Here is a closer look at our microscopy and other imaging tools for cell and tissue research available at the Advanced Cellular & Tissue Microscopy Core.
We offer a range of cutting-edge tools to meet your needs for tissue and cell imaging and examination.
Fluo View™ 1000 Confocal Microscope (R6-411)
The Olympus FV1000 is a next-generation imaging system designed for high-resolution, confocal observation of both fixed and living cells. The FV1000 offers advances in confocal system performance while providing the speed and sensitivity required for live cell imaging with minimal risk of damage to living specimens. In addition, the FV1000 offers a revolutionary synchronized laser scanning system called the SIM Scanner. While one laser stimulates, the second laser simultaneously provides high-resolution imaging. This coordination of laser stimulation and imaging makes the FV1000 an ideal choice for FRAP, FLIP and photo-activation.
The FV1000 includes five lasers: 405 Laser Diode; 458, 488, 515 mArgon; 543 HeNe; 633 HeNe; and Near-infrared LD748.
Live Cell Confocal Image System (R6-411)
The system consists of an IX81 automated inverted microscope, SRV CCD Digital Camera and CO2/humidity/temperature control incubator. It delivers an excellent environment for live cell phase contrast, differential interference contrast (DIC) and various fluorescent imaging scenarios. The filters sets are for DAPI, Hoechst and AMCA excitation; for FITC/EGFP/BODIPY/FUO3DIO excitation; and for TEXAS RED and CY5. The objectives are 4X, 10X, 20X, 40X and 40X, 60X and 100X resolutions for oil immersion microscopy.
Live Cell Spinning disk Confocal Image System (R6-411)
A spinning-disk (Nipkow disk) confocal microscope uses a series of moving pinholes on a disk to scan spot of light. Since a series of pinholes scans an area in parallel, each pinhole is allowed to hover over a specific area for a longer amount of time thereby reducing the excitation energy needed to illuminate a sample when compared with laser scanning microscopes. Decreased excitation energy reduces phototoxicity and photobleaching of a sample, which makes it ideal for imaging live cells or organisms. This system offers fully motorized disk control so a computer can easily engage the disk into the light path and select wavelengths through the included filter changer; this also makes it easier to switch between confocal and wide-field techniques. Image formation is obtained from an EM-CCD camera that allows full frame images to be acquired at up to 15 frames per second. The DSU is excellent for live-cell applications where speed of acquisition and minimal phototoxicity is paramount.
INCUCYTE™ Live-Cell Imaging System (R6-411)
IncuCyte allows you to place a microscope inside your incubator, taking advantage of an asset you already own as well as eliminating any potential problems that may result from disturbing cells during the observation process. This IncuCyte approach makes long-term kinetic imaging convenient and affordable. It provides a more efficient and thorough set of solutions for long-term, live-cell imaging. The IncuCyte is designed with increased productivity and supports hundreds of standard varieties of cell culture vessels, including 96- and 384-well plates, T-flasks, dishes and micro-slides. With three configurable trays, you can mix and match multiple vessels and even monitor different experiments running concurrently.
CRI Nuance Multispectral Imaging System (R6-412)
Nuance has two main advantages over confocal multispectral systems. It is able to do bright-field imaging of chromogenic molecular markers while the confocal imaging systems are restricted to fluorescence-based applications. Also, for fluorescence microscopy, in samples that are auto-fluorescent, Nuance is superior to most laser-scanning multispectral confocal systems at separating tissue auto-fluorescence from signals of interest. This is partly because of Nuance’s light-efficient CCD-based imaging system that is excellent for shot-noise limited samples and partly thanks to Nuance’s algorithms for determining the pure spectral signatures of fluorophores from tissue sections with auto-fluorescence.
Total Internal Reflection Fluorescence Microscopy (R6-412)
Total internal reflection microscopy (TIRFM) is an optical technique used to observe single molecule fluorescence. This technique is gaining popularity with cell biologists and neuroscientists to observe cell membrane fluorescence, in part because new membrane-specific dyes have been developed. Two laser lines are setup for TIFF imaging (using 458, 488, 515 mArgon and 543 HeNe lasers). Major advantages of TIRFM are that with it, you can visualize discreet structures and single molecules without interference from ubiquitous fluorophores and you get excellent signal-to-noise ratios. The environmental is fully controlled by the Tokai Hit Stage Top incubator, which provides accurate control of the temperature, CO2 and humidity for long-term time-lapse imaging of living cells and tissues.
Regular Inverted BF and Fluorescent Microscope (R6-412)
The IX81 begins with a unique two-tiered, V-shaped optical design that provides tremendous versatility without frame modifications and bright images with minimal reflections. An optional six-position fluorescence filter turret accepts a unique analyzer holder for switching between DIC/polarized light and fluorescence observations. With its built-in motorized Z-axis drive, six-position nosepiece and light path selector, the IX81 is ready for multi-wavelength, advanced fluorescence and de-convolution techniques.
Regular Upright BF and Fluorescent Microscope (R6-411)
The BX61’s completely modular frame and optical design provides a flexible, fast, reliable and precise upright BF/fluorescent imaging system. It has been recently upgraded with reflecting imaging.
Nikon A1 Confocal Imaging System (R9-317)
The Nikon A1 Confocal Imaging System has a Hybrid Confocal Scan Head that enhances the optical efficiency and minimizes light loss. It can perform ultra-high-speed imaging at 420 fps (512x32 pixels) using a resonant scanner with a resonance frequency of 7.8kHz; the system provides fast spectral imaging at 16 fps (512x64 pixels) and real-time spectral unmixing. With average light transmission of 98 percent, it can deliver up to a 30 percent increase in fluorescence detection efficiency.
Laser Capture Micro-dissection (LCM, Arcturus) (R6-412)
This Laser Capture Micro-dissection (LCM) method is extraordinarily gentle and ideal for micro-dissection of single cells or small numbers of cells. The ArcturusXT™ micro-dissection instruments each combine LCM and UV laser cutting for ultimate micro-dissection flexibility. These instruments remove the guesswork from the micro-dissection process by allowing researchers to maintain custody of the sample throughout the experiment, ensuring that only the desired material has been collected.
ImageStreamX High Resolution Microscopy in Flow (R6-412)
The ImageStreamX combines the high-speed capture of high-resolution imagery with detailed quantitation to create a statistically robust microscopy platform for a wide range of cell analysis applications. ImageStreamX produces up to 12 high resolution images of each cell directly in flow, at rates exceeding 1,000 cells per second with the fluorescence sensitivity of the best conventional flow cytometers. These breakthrough capabilities allow you to quantitate cellular morphology and the intensity and location of fluorescent probes on, in or between cells, even in rare sub-populations and highly heterogeneous samples.
With this system, you can image cells directly in suspension with the resolution of a 60X microscope and the fluorescence sensitivity of the best flow cytometers. You can analyze highly heterogeneous samples and rare cell sub-populations at speeds exceeding 1,000 cells per second, as well as perform phenotypic and functional studies at the same time using up to five lasers and 12 images per cell. The system will help you quantitate virtually anything you can see using the IDEAS® software package’s numerous pre-defined fluorescence and morphologic parameters.
We offer peripheral equipment to support our microscope and imaging systems:
- Live cell microscopy environmental control system
- Tissue culture incubator (37°C with 5 percent CO2) for short-term storage
- Dell Precision 390 workstation
- Olympus DP-BSW image capture and control software
- Olympus LSM Software package
- Intelligent Imaging Innovations’ SlideBook Software package for 2-D and 3-D imaging on PC computer platforms
For customized software development of specialized bioassays, please contact Dr. Stephen Wong.